Starkie R L, Rolland J, Angus D J, Anderson M J, Febbraio M A
Exercise Physiology and Metabolism Laboratory, Department of Physiology, University of Melbourne, Parkville 3052, Victoria, Australia.
Am J Physiol Cell Physiol. 2001 Apr;280(4):C769-74. doi: 10.1152/ajpcell.2001.280.4.C769.
The present study was undertaken to examine the effect of prolonged running on monocyte intracellular cytokine production and plasma cytokine concentration. Blood samples were collected 1 h before, immediately after, 2 h after, and 24 h after a competitive marathon run. There was no change in the number of cells spontaneously producing tumor necrosis factor (TNF)-alpha; however, there was a decrease in the number of cells producing interleukin (IL)-1alpha and IL-6 (P < 0.01) postexercise. In contrast, there was an increase in the number of monocytes that responded to lipopolysaccharide stimulation by producing IL-1alpha, TNF-alpha, and IL-6 (P < 0.01) immediately and 2 h postexercise; however, these cells contained less cytokine (P < 0.05). Plasma IL-6, TNF-alpha, epinephrine, norepinephrine, and cortisol concentrations were markedly increased (P < 0.01) postexercise. These data demonstrate that circulating monocytes are not the source of elevated levels of plasma IL-6 and TNF-alpha after prolonged running. In addition, it is likely that stress hormones result in a decrease in the amount of cytokine produced by LPS-stimulated cells postexercise.
本研究旨在探讨长时间跑步对单核细胞细胞内细胞因子产生及血浆细胞因子浓度的影响。在一场马拉松比赛前1小时、比赛结束后即刻、2小时及24小时采集血样。自发产生肿瘤坏死因子（TNF）-α的细胞数量没有变化；然而，运动后产生白细胞介素（IL）-1α和IL-6的细胞数量减少（P<0.01）。相反，运动后即刻及2小时，对脂多糖刺激产生IL-1α、TNF-α和IL-6的单核细胞数量增加（P<0.01）；然而，这些细胞所含细胞因子较少（P<0.05）。运动后血浆IL-6、TNF-α、肾上腺素、去甲肾上腺素和皮质醇浓度显著升高（P<0.01）。这些数据表明，长时间跑步后血浆IL-6和TNF-α水平升高的来源并非循环单核细胞。此外，应激激素可能导致运动后脂多糖刺激细胞产生的细胞因子量减少。
