Deutsch W A, Dorson J W, Moses R E
J Bacteriol. 1976 Jan;125(1):220-4. doi: 10.1128/jb.125.1.220-224.1976.
Toluene-treated cells were used for examining excision of pyrimidine dimers in Escherichia coli strains W3110, DM845 (uvrA-), P3478 (polA-), and KS5064 (polAex1). Excision occurring in toluene-treated cells is rapid, adenosine 5'-triphosphate dependent, and requires the uvrA gene function. In strains lacking either the polymerizing or 5' leads to 3' exonucleolytic activity of deoxyribonucleic acid polymerase I, excision does occur. However, both in vivo and in vitro, the excision in such strains is initially slower than wild type.
用甲苯处理过的细胞来检测大肠杆菌菌株W3110、DM845(uvrA-)、P3478(polA-)和KS5064(polAex1)中嘧啶二聚体的切除情况。在甲苯处理过的细胞中发生的切除过程迅速,依赖于三磷酸腺苷,并且需要uvrA基因功能。在缺乏DNA聚合酶I的聚合或5'至3'核酸外切酶活性的菌株中,切除确实会发生。然而,无论在体内还是体外,此类菌株中的切除最初都比野生型慢。