三磷酸腺苷（ATP）在大肠杆菌紫外线辐射损伤切除修复中的作用
Role of ATP in excision repair of ultraviolet radiation damage in Escherichia coli.
作者信息
Waldstein E A, Sharon R, Ben-Ishai R
出版信息
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2651-4. doi: 10.1073/pnas.71.7.2651.
Abstract
The effect of ATP on the first step of excision repair of ultraviolet damage in DNA has been studied using toluene-treated E. coli. During postirradiation incubation, five to six times more single-strand breaks are formed in DNA in the presence of exogenous ATP than in its absence. The ATP-dependent as well as the ATP-independent endonucleolytic activities appear to be catalyzed by the same enzyme since both activities are almost completely absent in uvrA and uvrB mutants. An ATP-dependent endonucleolytic activity has been detected in nonirradiated toluene-treated E. coli. It is concluded that ATP is required in vivo for either the incision step of repair or an enzymatic reaction preceding it.
摘要
利用经甲苯处理的大肠杆菌，研究了ATP对DNA中紫外线损伤切除修复第一步的影响。在照射后的孵育过程中，存在外源性ATP时，DNA中形成的单链断裂比不存在时多五到六倍。依赖ATP的以及不依赖ATP的内切核酸酶活性似乎由同一种酶催化，因为在uvrA和uvrB突变体中这两种活性几乎完全缺失。在未照射的经甲苯处理的大肠杆菌中检测到了依赖ATP的内切核酸酶活性。得出的结论是，体内修复的切口步骤或其之前的酶促反应需要ATP。
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