Görür Kemal, Ozcan Cengiz, Polat Ayse, Unal Murat, Tamer Lülüfer, Cinel Ismail
Department of Otorhinolaryngology, Mersin University Faculty of Medicine, Turkey.
J Laryngol Otol. 2002 Jun;116(6):426-9. doi: 10.1258/0022215021911202.
The possible effect of selenium on the prevention or reduction in occurrence of myringosclerosis was investigated. Fifteen rats were myringotomized bilaterally and separated into two groups. Nine rats were treated with selenium in the study group. Six rats were administered physiological serum and formed the control group. The occurrence of myringosclerotic lesions and anti-apoptotic activity in the tympanic membranes of the two groups were compared otomicroscopically and histopathologically. The sclerosis was occasional in three, moderate in five and severe in three tympanic membranes in the control group. On the other hand sclerosis was observed in only two of 18 specimens in the study group and sclerosis was seen only occasionally in these two sections. Although Bcl-2 staining, which indicates apoptosis, was not statistically different between the groups, it was observed that apoptosis was slightly more apparent in the study group (eight of 18 tympanic membranes versus two of 12 tympanic membranes in the control group). In conclusion, the formation of myringosclerosis following myringtomoy in rats can be reduced by intraperitoneal selenium administration.
研究了硒对预防或减少鼓膜硬化症发生的可能作用。对15只大鼠进行双侧鼓膜切开术，并将其分为两组。研究组的9只大鼠接受硒治疗。6只大鼠给予生理血清，构成对照组。通过耳显微镜检查和组织病理学比较两组鼓膜中鼓膜硬化性病变的发生情况和抗凋亡活性。对照组中，12个鼓膜硬化偶尔出现3例，中度出现5例，重度出现3例。另一方面，研究组18个标本中仅2个观察到硬化，且这两个切片中仅偶尔见到硬化。虽然两组之间表明细胞凋亡的Bcl-2染色在统计学上无差异，但观察到研究组细胞凋亡略更明显（研究组18个鼓膜中有8个，对照组12个鼓膜中有2个）。总之，腹腔注射硒可减少大鼠鼓膜切开术后鼓膜硬化的形成。
