Sekine T, Nakamura T, Shimizu Y, Ueda H, Matsumoto K, Takimoto Y, Kiyotani T
Department of Bioartificial Organs, Field of Clinical Application, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Kyoto 606-8397, Japan.
J Biomed Mater Res. 2001 Feb;54(2):305-10. doi: 10.1002/1097-4636(200102)54:2<305::aid-jbm18>3.0.co;2-b.
We have developed a new adhesive for surgical use. The new adhesive is made of three components: porcine collagen, poly(L-glutamic acid) and water-soluble carbodiimides (WSC). The optimum concentration of each component was determined by measuring the time required for gel formation in experiments in vitro. Using these optimum concentrations, we applied the adhesive to wounds made on rats. A conventional fibrin glue was used as a control. Measurement of tensile strength and histological examination were performed 5, 7, 10, and 14 days after the operation. The tensile strength of wounds treated with 2.5 mg/mL collagen glue was not significantly different from that of wounds treated with fibrin glue except at 7 days after the operation (p < 0.05 by Student's t-test). Histological examination revealed that the speed of cell infiltration into, and absorption of 2.5 mg/mL collagen glue was slower than for fibrin glue, but faster than for 5.0 mg/mL collagen glue. One of the important advantages of our collagen glue is that the absorption rate of it can be controlled by the collagen concentration. Therefore, it seems to be adequate for sealing air leakage from the lung, which takes a relatively long period for recovery. Moreover it does not contain human serum, and, hence, it requires no blood donation and can be obtained with low cost.
我们研发了一种新型外科用粘合剂。这种新型粘合剂由三种成分制成：猪胶原蛋白、聚（L-谷氨酸）和水溶性碳二亚胺（WSC）。通过在体外实验中测量凝胶形成所需的时间来确定每种成分的最佳浓度。使用这些最佳浓度，我们将粘合剂应用于大鼠身上制造的伤口。使用传统的纤维蛋白胶作为对照。在手术后5、7、10和14天进行拉伸强度测量和组织学检查。除了手术后7天外，用2.5mg/mL胶原蛋白胶治疗的伤口的拉伸强度与用纤维蛋白胶治疗的伤口的拉伸强度没有显著差异（通过学生t检验，p<0.05）。组织学检查显示，细胞浸润到2.5mg/mL胶原蛋白胶中以及对其吸收的速度比纤维蛋白胶慢，但比5.0mg/mL胶原蛋白胶快。我们的胶原蛋白胶的一个重要优点是其吸收率可以通过胶原蛋白浓度来控制。因此，它似乎足以用于封闭肺部的漏气，肺部漏气恢复需要相对较长的时间。此外，它不含人血清，因此不需要献血，并且可以低成本获得。
