Nam Y K, Cho Y S, Kim D S
Research Center for Ocean and Industrial Development, Pukyong National University, Pusan, South Korea.
Transgenic Res. 2000 Dec;9(6):463-9. doi: 10.1023/a:1026596422225.
As a model system for vertebrate transgenesis, fish have many attractive advantages, especially with respect to the characteristics of eggs, allowing us to produce isogenic, transgenic, homozygous vertebrates by combining with chromosome-set manipulation. Here, we describe the large-scale production of isogenic transgenic homozygous animals using our experimental organism, the mud loach Misgurnus mizolepis, by the simple process of artificial parthenogenesis in a single generation. These isogenic fish have retained transgenic homozygous status in a stable manner during the subsequent 5 years, and exhibited increased levels of transgene expression. Furthermore, their isogenic nature was confirmed by cloned transgenic homozygous offspring produced via another step of parthenogenic reproduction of the isogenic homozygous transgenic fish. These results demonstrate that a combination of transgenesis and artificial parthenogenesis will make the rapid utilization of genetically pure homozygous transgenic system in vertebrate transgenesis possible.
作为脊椎动物转基因的模型系统，鱼类具有许多吸引人的优势，特别是在卵的特性方面，这使我们能够通过结合染色体组操作来产生同基因、转基因的纯合脊椎动物。在此，我们描述了利用我们的实验生物——泥鳅（Misgurnus mizolepis），通过单代人工孤雌生殖的简单过程大规模生产同基因转基因纯合动物。这些同基因鱼在随后的5年中以稳定的方式保持转基因纯合状态，并表现出转基因表达水平的提高。此外，通过同基因纯合转基因鱼的另一步孤雌生殖产生的克隆转基因纯合后代证实了它们的同基因性质。这些结果表明，转基因与人工孤雌生殖的结合将使脊椎动物转基因中遗传纯合转基因系统的快速利用成为可能。
