Wang J N, Chen Y J, Hano Y, Nomura T, Tan R X
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
Acta Pharmacol Sin. 2000 Jul;21(7):633-6.
To study the antioxidant action of five polyphenols (+) catechin, procyanidin B2, procyanidin B5, procyanidin B5 3'-O-gallate, and amurensisin isolated from the seeds of Vitis amurensis.
The mouse liver homogenate lipid peroxidation assay was applied for the evaluation of the antioxidant activity in vitro.
(+) Catechin, procyanidin B2, procyanidin B5, procyanidin B5 3'-O-gallate, and amurensisin showed antioxidant activity with the IC50 values of 0.47, 0.25, 0.10, 0.02, and 0.03 mmol/L, respectively. The IC50 value of vitamin E used as a positive control was 0.13 mmol/L. The structural activity relationship was also analyzed. Procyanidins carrying a galloyl group possessed higher anti-lipid peroxidation activities. All dimers were found to be more potent than the non-galloylated momomer such as (+) catechin. However, the activity of the 4-->6 linked dimer seemed more preferable than 4-->8 linked dimer.
Procyanidin B5, procyanidin B5 3'-O-gallate, and amurensisin showed a more antioxidant activity than vitamin E did, and their activity is dependent on their substitution and polymerization patterns.
研究从山葡萄种子中分离出的五种多酚（+）儿茶素、原花青素B2、原花青素B5、原花青素B5 3'-O-没食子酸酯和山葡萄素的抗氧化作用。
采用小鼠肝脏匀浆脂质过氧化测定法体外评价抗氧化活性。
（+）儿茶素、原花青素B2、原花青素B5、原花青素B5 3'-O-没食子酸酯和山葡萄素均表现出抗氧化活性，IC50值分别为0.47、0.25、0.10、0.02和0.03 mmol/L。用作阳性对照的维生素E 的IC50值为0.13 mmol/L。还分析了构效关系。带有没食子酰基的原花青素具有较高的抗脂质过氧化活性。所有二聚体均比非没食子酰化的单体如（+）儿茶素更有效。然而，4→6连接二聚体的活性似乎比4→8连接二聚体更优。
原花青素B5、原花青素B5 3'-O-没食子酸酯和山葡萄素表现出比维生素E更强的抗氧化活性, 且它们 的活性取决于其取代和聚合模式。
