Scazzocchio F, Cometa M F, Tomassini L, Palmery M
Planta Med. 2001 Aug;67(6):561-4. doi: 10.1055/s-2001-16493.
The antibacterial activity of extract and isolated major alkaloids (berberine, beta-hydrastine, canadine and canadaline) of Hydrastis canadensis L. (Ranunculaceae) was evaluated against 6 strains of microorganism: Staphylococcus aureus (ATCC 25 993 and ATCC 6538P), Streptococcus sanguis (ATCC 10 556), Escherichia coli (ATCC 25 922), Pseudomonas aeruginosa (ATCC 27 853). Bactericidal activity was evaluated by contact test by measuring the "killing time" on a low density bacterial inoculum, and bacteriostatic activity in liquid medium by M.I.C. values. The results provide a rational basis for the traditional antibacterial use of Hydrastis canadensis.
对毛茛科植物北美黄连（Hydrastis canadensis L.）提取物及分离得到的主要生物碱（小檗碱、β-白毛茛碱、加拿大黄连碱和加拿大豆碱）针对6种微生物菌株的抗菌活性进行了评估，这6种微生物菌株分别为：金黄色葡萄球菌（ATCC 25993和ATCC 6538P）、血链球菌（ATCC 10556）、大肠杆菌（ATCC 25922）、铜绿假单胞菌（ATCC 27853）。通过接触试验，通过测量低密度细菌接种物的“杀菌时间”来评估杀菌活性，并通过最低抑菌浓度（M.I.C.）值在液体培养基中评估抑菌活性。这些结果为北美黄连传统的抗菌用途提供了合理依据。
