Zanetti F, Mattiello R, Garbino C, Kaloghlian A, Terrera M V, Boviez J, Palma E, Carrillo E, Berinstein A
Instituto de Biotecnología, CNIA, INTA, Castelar, Buenos Aires, Argentina.
Avian Dis. 2001 Jul-Sep;45(3):567-71.
In this report, we describe the biological and molecular characterization of a paramyxovirus type-1 (PPMV-1) isolate found in wild pigeons in an urban habitat in Buenos Aires, Argentina. Of the nine pigeons captured, three were moribund, and the other six showed diarrhea, ataxia, tremor, torticolis, and wing paralysis. The intracerebral pathogenicity index was 1.29, and the amino acid (aa) sequence at the fusion protein cleavage site was 112GRQ KRF117. These characteristics correspond to a virulent Newcastle disease virus isolate. Nevertheless, it was not possible to reproduce the disease in chickens experimentally although the chickens exhibited seroconversion after inoculation. On the other hand, pigeons inoculated with the isolate became sick. These results provide further evidence about the unusual pathogenicity of PPMV-1 for chickens and show once more the need for more biological determinations in these cases to arrive at a final conclusion.
在本报告中，我们描述了在阿根廷布宜诺斯艾利斯一个城市栖息地的野生鸽子中发现的1型副粘病毒（PPMV-1）分离株的生物学和分子特征。在捕获的9只鸽子中，3只濒死，另外6只出现腹泻、共济失调、震颤、斜颈和翅膀麻痹。脑内致病性指数为1.29，融合蛋白裂解位点的氨基酸（aa）序列为112GRQ KRF117。这些特征与强毒新城疫病毒分离株相符。然而，尽管鸡接种后出现血清转化，但无法通过实验在鸡身上复制该疾病。另一方面，接种该分离株的鸽子发病。这些结果为PPMV-1对鸡的异常致病性提供了进一步证据，并再次表明在这些情况下需要进行更多生物学测定才能得出最终结论。
