Werner G, Cuny C, Schmitz F J, Witte W
Wernigerode Branch, Robert Koch Institute, D-38855 Wernigerode, Germany.
J Clin Microbiol. 2001 Oct;39(10):3586-90. doi: 10.1128/JCM.39.10.3586-3590.2001.
Of 3,052 Staphylococcus aureus strains collected by the European SENTRY surveillance study, 35 were found to be nonsusceptible to quinupristin-dalfopristin (MIC of > or =2 mg/liter). These isolates originated from four hospitals in France and one in Spain. In isolates from two Parisian hospitals exhibiting the same SmaI macrorestriction pattern, streptogramin resistance was based on vatA and vgbA. One isolate from a hospital in Lyon and 22 from a hospital in Lille were of the vatB vgaB streptogramin A resistance genotype and possessed ermA and/or ermC. As deduced from the loss of either streptogramin A or streptogramin B resistance determinants in particular isolates, resistance to quinupristin-dalfopristin requires mechanisms conferring resistance to both compounds. The SmaI macrorestriction patterns of strains from hospitals in Lille and Lyon were different; however, similarity analysis suggested a relatedness of 20 methicillin-resistant S. aureus strains from the Lille hospital, a finding confirmed by PCR typing based on three different genomic polymorphisms. These groups of isolates were found to be hetero-glycopeptide-intermediate susceptible S. aureus. Information about the failure of glycopeptide chemotherapy has not been available.
在欧洲哨兵监测研究收集的3052株金黄色葡萄球菌菌株中，发现35株对奎奴普丁 - 达福普汀不敏感（最低抑菌浓度≥2毫克/升）。这些分离株分别来自法国的四家医院和西班牙的一家医院。在来自两家巴黎医院且具有相同SmaI酶切图谱的分离株中，链阳性菌素耐药性基于vatA和vgbA。来自里昂一家医院的1株分离株和里尔一家医院的22株分离株具有vatB vgaB链阳性菌素A耐药基因型，并拥有ermA和/或ermC。从特定分离株中链阳性菌素A或链阳性菌素B耐药决定簇的缺失推断，对奎奴普丁 - 达福普汀的耐药性需要同时具备对两种化合物的耐药机制。里尔和里昂医院菌株的SmaI酶切图谱不同；然而，相似性分析表明，里尔医院的20株耐甲氧西林金黄色葡萄球菌菌株具有相关性，基于三种不同基因组多态性的PCR分型证实了这一发现。这些分离株群体被发现是异质性糖肽中介敏感金黄色葡萄球菌。关于糖肽类化疗失败的信息尚无报道。
