Robak E, Niewiadomska H, Robak T, Bartkowiak J, Błoński J Z, Woźniacka A, Pomorski L, Sysa-Jedrezejowska A
Department of Dermatology and Venereology, University of Lódz, Poland.
Mediators Inflamm. 2001 Aug;10(4):179-89. doi: 10.1080/09629350124724.
Human Tgammadelta lymphocytes constitute from 1 to 15% of all peripheral blood lymphocytes. Recent work has demonstrated that this population plays a major role in the pathogenesis of infectious and immune diseases. Increased numbers of gammadelta T cells have been found in affected skin from systemic sclerosis and chronic cutaneous lupus erythematosus patients. In our study, we have determined the numbers of Tgammadelta lymphocytes and their subpopulations in peripheral blood from 29 patients with systemic lupus erythematosus (SLE) and in 19 healthy volunteers using flow cytometry and specific monoclonal antibodies. The same cells in uninvolved skin from SLE patients and human controls using immunohistochemical analysis were estimated. T-Cell receptor (TCR) delta chain gene rearrangement was identified with primers for Vdelta1, Vdelta2 and Vdelta3 by the polymerase chain reaction. Statistical analysis showed a significantly decreased number of gammadelta T cells in SLE patients (26.4+/-16.9/microl) compared with the control group (55.3+/-20.6/microl (p < 0.001). The number of Vdelta2 TCR+ and Vgamma9 TCR+ subpopulations was also lower in SLE patients than in healthy persons. No statistical correlation between disease activity and the number of gammadelta T cells was demonstrated. The percentage of Tgammadelta lymphocytes in clinically normal skin from SLE patients was twice (22.0+/-9.4%) that found in the skin from healthy persons (11.1+/-5.5%) (p < 0.002). Higher percentages of the Vdelta2 TCR+ and Vgamma9 TCR+ subpopulation of lymphocytes were found in the skin from SLE patients. We have also found positive correlation between the percentage of Tgammadelta lymphocytes in skin and the activity of SLE (r=0.594, p < 0.001), and between subpopulation Vdelta3 TCR+ and disease activity (r=0.659, p< 0.001). In conclusion, the results of our studies demonstrate that, in patients with SLE, accumulation of Tgammadelta lymphocytes can be seen in clinically normal skin, and the percentage of these cells correlates with the activity of the disease.
人类γδ T淋巴细胞占外周血淋巴细胞总数的1%至15%。最近的研究表明，这一细胞群体在感染性疾病和免疫性疾病的发病机制中起主要作用。在系统性硬化症和慢性皮肤性红斑狼疮患者的病变皮肤中，已发现γδ T细胞数量增加。在我们的研究中，我们使用流式细胞术和特异性单克隆抗体，测定了29例系统性红斑狼疮（SLE）患者外周血中γδ T淋巴细胞及其亚群的数量，并与19名健康志愿者进行了比较。同时，通过免疫组织化学分析，对SLE患者和健康对照者未受累皮肤中的相同细胞进行了评估。通过聚合酶链反应，使用针对Vδ1、Vδ2和Vδ3的引物，鉴定了T细胞受体（TCR）δ链基因重排。统计分析显示，与对照组（55.3±20.6/μl，p<0.001）相比，SLE患者的γδ T细胞数量显著减少（26.4±16.9/μl）。SLE患者中Vδ2 TCR+和Vγ9 TCR+亚群的数量也低于健康人。未发现疾病活动度与γδ T细胞数量之间存在统计学相关性。SLE患者临床正常皮肤中γδ T淋巴细胞的百分比（22.0±9.4%）是健康人皮肤中（11.1±5.5%）的两倍（p<0.002）。在SLE患者的皮肤中，淋巴细胞Vδ2 TCR+和Vγ9 TCR+亚群的百分比更高。我们还发现皮肤中γδ T淋巴细胞的百分比与SLE的活动度之间存在正相关（r=0.594，p<0.001），以及Vδ3 TCR+亚群与疾病活动度之间存在正相关（r=0.659，p<0.001）。总之，我们的研究结果表明，在SLE患者中，在临床正常皮肤中可观察到γδ T淋巴细胞的积聚，且这些细胞的百分比与疾病活动度相关。
