Lorenzo S, Iglesias R, Paniagua E, Ansotegui I, Alonso J M, Ubeira F M
Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.
Med Microbiol Immunol. 2001 Sep;189(4):177-83. doi: 10.1007/s004300100065.
Biotinyl-enzymes are conservative molecules present in helminths, as well as in other animals, bacteria and plants. They have recently been found to be antigenic in mice, and a potential source of cross-reactivity among helminths. This study investigated the presence in human sera of antibodies reactive with biotinyl-enzymes from the nematodes Anisakis simplex, Toxocara canis and Ascaris suum. Biotinyl-enzymes from all these nematodes were recognized by IgG1 antibodies in sera from healthy subjects and from Anisakis-free patients infected with other parasites. Interestingly, IgE antibodies reactive with Anisakis simplex biotinyl-enzymes were present in about one third of the sera from Anisakis-free patients infected with other parasites. Our results also demonstrate that the anti-BE IgG1 and IgE antibodies present in the sera of Anisakis-free subjects are cross-reactive among helminths. We conclude that biotinyl-enzymes from nematodes are recognized by natural human antibodies, although Anisakis biotinyl-enzymes do not seem to be the cause of sensitization. Since sera from the Anisakis-free population also present these antibodies, as-yet unidentified factors (dietary components, intestinal inflammation and/or the presence of parasites) may contribute to the induction of anti-BE antibody background.
生物素化酶是存在于蠕虫以及其他动物、细菌和植物中的保守分子。最近发现它们在小鼠中具有抗原性，并且是蠕虫之间潜在的交叉反应源。本研究调查了人类血清中与来自线虫简单异尖线虫、犬弓首蛔虫和猪蛔虫的生物素化酶发生反应的抗体的存在情况。来自所有这些线虫的生物素化酶在健康受试者以及感染其他寄生虫的无异尖线虫患者的血清中被IgG1抗体识别。有趣的是，在感染其他寄生虫的无异尖线虫患者的血清中，约三分之一存在与简单异尖线虫生物素化酶发生反应的IgE抗体。我们的结果还表明，无异尖线虫受试者血清中存在的抗生物素化酶IgG1和IgE抗体在蠕虫之间具有交叉反应性。我们得出结论，线虫的生物素化酶可被天然人类抗体识别，尽管异尖线虫生物素化酶似乎不是致敏原因。由于无异尖线虫人群的血清中也存在这些抗体，尚未确定的因素（饮食成分、肠道炎症和/或寄生虫的存在）可能有助于诱导抗生物素化酶抗体背景。
