Lynch H J, Wurtman R J, Moskowitz M A, Archer M C, Ho M H
Science. 1975 Jan 17;187(4172):169-71. doi: 10.1126/science.1167425.
The melatonin in urine samples from six healthy adult volunteers was concentrated on Amberlite XAD-2 resin, eluted with organic solvents, and quantitated by use of a bioassay technique (the dermal melanaphore response of larval anurans to melatonin in their bathing medium). The melatonin content of samples collected between 11 p.m. and 7 a.m. was, in each case, several times higher than that of samples collected between 7 a.m. and 3 p.m. or between 3 p.m. and 11 p.m.
来自六名健康成年志愿者的尿液样本中的褪黑素，被浓缩在Amberlite XAD - 2树脂上，用有机溶剂洗脱，并通过一种生物测定技术（幼体无尾两栖动物的皮肤黑素细胞对其浴液中褪黑素的反应）进行定量分析。在晚上11点至早上7点之间采集的样本中的褪黑素含量，在每种情况下，都比在早上7点至下午3点之间或下午3点至晚上11点之间采集的样本高出几倍。
