Liu H, Sachidanandam R, Stein L
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Genome Res. 2001 Dec;11(12):2020-6. doi: 10.1101/gr.194501.
We have investigated possible collinearity between the genomes of rice and Arabidopsis by comparing 126 annotated and mapped rice BAC sequences (approximately 20 Mb of sequence) with the annotated and complete Arabidopsis genome (approximately 115 Mb). Although we were able to identify several regions in which gene order is preserved, they are relatively small, and are interrupted by noncollinear genes. Computer simulation showed that these microscale collinearities are above the expectation for a random process. On the other hand, the order of exons within homologous genes (<2.5 kb) was preserved, as expected.
我们通过将126个注释和定位的水稻BAC序列(约20 Mb序列)与注释完整的拟南芥基因组(约115 Mb)进行比较,研究了水稻和拟南芥基因组之间可能存在的共线性。尽管我们能够识别出几个基因顺序得以保留的区域,但这些区域相对较小,并且被非共线基因打断。计算机模拟表明,这些微观尺度的共线性高于随机过程的预期。另一方面,正如预期的那样,同源基因(<2.5 kb)内的外显子顺序得以保留。