Moore J W, Ramón F, Joyner R W
Biophys J. 1975 Jan;15(1):25-35. doi: 10.1016/S0006-3495(75)85789-4.
This is the second in a series of four papers on the simulation of the voltage clamp of cylindrical excitable cells. In this paper we evaluate the double sucrose-gap voltage-clamp technique for the squid and lobster giant axons. Using the Crank-Nicolson method of solution of the cable equations and differential equations representing the voltage clamp circuit we studied the effect of length of the sucrose gap "node" on the voltage profile along an excitable cell during a simulated voltage clamp. The voltage gradients along the region of the cell within the node produce "notches" in the current recording as well as changes in the magnitude of the sodium and potassium current for a given voltage step. Our results show that good voltage clamp control requires node lengths less than one-half the axon diameter.
这是关于圆柱形可兴奋细胞电压钳模拟的四篇系列论文中的第二篇。在本文中，我们评估了用于鱿鱼和龙虾巨轴突的双蔗糖间隙电压钳技术。使用求解电缆方程和代表电压钳电路的微分方程的克兰克 - 尼科尔森方法，我们研究了在模拟电压钳期间，蔗糖间隙“节点”的长度对沿可兴奋细胞的电压分布的影响。节点内细胞区域的电压梯度会在电流记录中产生“凹口”，并且对于给定的电压阶跃，钠电流和钾电流的大小也会发生变化。我们的结果表明，良好的电压钳控制要求节点长度小于轴突直径的一半。
