Perilli Mariagrazia, Dell'Amico Emanuela, Segatore Bernardetta, de Massis Maria Rosaria, Bianchi Ciro, Luzzaro Francesco, Rossolini Gian Maria, Toniolo Antonio, Nicoletti Giuseppe, Amicosante Gianfranco
Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, L'Aquila, Italy.
J Clin Microbiol. 2002 Feb;40(2):611-4. doi: 10.1128/JCM.40.2.611-614.2002.
Extended-spectrum beta-lactamases (ESBLs) are widespread in hospital settings worldwide. The present investigation was undertaken to assess the distribution and prevalence of ESBLs belonging to the TEM and SHV families in 448 ESBL-producing clinical isolates of Enterobacteriaceae collected from 10 different Italian hospitals. The natures of TEM and SHV determinants were identified by direct sequencing of PCR-amplified genes. TEM-52 and SHV-12 were the most common variants, and they were found in most hospitals and in several different species. Other less frequent variants included TEM-5, TEM-12, TEM-15, TEM-19, TEM-20, TEM-24, TEM-26, TEM-43, TEM-60, TEM-72, TEM-87, SHV-2a, SHV-5, and SHV-11. Proteus mirabilis was the most common producer of TEM-type ESBLs, while Klebsiella pneumoniae was the most common producer of SHV-type ESBLs. The distribution of TEM- and SHV-type ESBL variants in Enterobacteriaceae from Italian hospitals exhibited notable differences from those from other geographical settings.
超广谱β-内酰胺酶（ESBLs）在全球医院环境中广泛存在。本研究旨在评估从意大利10家不同医院收集的448株产ESBLs的肠杆菌科临床分离株中属于TEM和SHV家族的ESBLs的分布及流行情况。通过对PCR扩增基因进行直接测序来鉴定TEM和SHV决定簇的性质。TEM-52和SHV-12是最常见的变体，在大多数医院和几种不同的菌种中均有发现。其他不太常见的变体包括TEM-5、TEM-12、TEM-15、TEM-19、TEM-20、TEM-24、TEM-26、TEM-43、TEM-60、TEM-72、TEM-87、SHV-2a、SHV-5和SHV-11。奇异变形杆菌是TEM型ESBLs最常见的产生菌，而肺炎克雷伯菌是SHV型ESBLs最常见的产生菌。意大利医院肠杆菌科中TEM型和SHV型ESBL变体的分布与其他地理区域存在显著差异。
