用半乳糖氧化酶处理人淋巴细胞对干扰素产生的酶促诱导作用。
Enzymatic induction of interferon production by galactose oxidase treatment of human lymphoid cells.
作者信息
Dianzani F, Monahan T M, Scupham A, Zucca M
出版信息
Infect Immun. 1979 Dec;26(3):879-82. doi: 10.1128/iai.26.3.879-882.1979.
Abstract
Human lymphocyte cultures produced large amounts of interferon after treatment with the enzyme galactose oxidase. Interferon production was detectable as early as 3 h after enzymatic treatment and reached a level of about 10(4) reference units 20 to 24 h later. Galactose oxidase-induced interferon appeared to be immune interferon on the basis of acid lability, lack of neutralization by antibody to leukocyte interferon, and slow kinetics of activation of the cellular antiviral state. Interferon production was inhibited to the same extent (99%) by pretreatment of the cells with beta-galactosidase or with neuraminidase followed by beta-galactosidase, suggesting that the critical event for activation of interferon production is the oxidation of exposed galactose residues on lymphocyte membrane.
摘要
用半乳糖氧化酶处理后，人淋巴细胞培养物产生了大量干扰素。酶处理后3小时即可检测到干扰素产生，20至24小时后达到约10⁴参考单位的水平。基于酸不稳定性、抗白细胞干扰素抗体不能中和以及细胞抗病毒状态激活的缓慢动力学，半乳糖氧化酶诱导的干扰素似乎是免疫干扰素。用β-半乳糖苷酶或神经氨酸酶预处理细胞，然后再用β-半乳糖苷酶处理，干扰素产生受到相同程度（99%）的抑制，这表明激活干扰素产生的关键事件是淋巴细胞膜上暴露的半乳糖残基的氧化。
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