Romero Gustavo Adolfo Sierra, Ishikawa Edna, Cupolillo Elisa, Toaldo Cristiane Bentin, Guerra Marcus Vinitius de Farias, Paes Marcilene Gomes, Macêdo Vanize de Oliveira, Shaw Jeffrey J
Núcleo de Medicina Tropical, Universidade de Brasília, Campus Universitário, Asa Norte. Caixa Postal 04517, DF, 70919-970, Brasilia, Brazil.
Acta Trop. 2002 Apr;82(1):25-9. doi: 10.1016/s0001-706x(02)00002-5.
Eighty Leishmania isolates from patients who contracted cutaneous leishmaniasis in the Manaus region, Amazonas State, Brazil, were identified as Leishmania (Viannia) guyanensis by the electrophoretic profiles of six enzymes. None reacted with the species-specific monoclonal antibody B19. Two L. (V.) guyanensis subpopulations were detected with the monoclonals B2 and B12. The lack of B19 expression by the L. (V.) guyanensis strains in the present study contrasts with that of the vast majority of the strains of the same parasite from eastern Amazonia and French Guyana that express the epitope.
通过六种酶的电泳图谱，从巴西亚马孙州马瑙斯地区感染皮肤利什曼病的患者中分离出的80株利什曼原虫被鉴定为圭亚那利什曼原虫（维阿尼利什曼原虫亚属）。没有一株与种特异性单克隆抗体B19发生反应。用单克隆抗体B2和B12检测到两个圭亚那利什曼原虫（维阿尼利什曼原虫亚属）亚群。本研究中圭亚那利什曼原虫（维阿尼利什曼原虫亚属）菌株缺乏B19表达，这与来自亚马孙东部和法属圭亚那的绝大多数表达该表位的同一寄生虫菌株形成对比。
