一步法PCR鉴别B病毒与相关灵长类α疱疹病毒。
One-step PCR to distinguish B virus from related primate alphaherpesviruses.
作者信息
Hirano Makoto, Nakamura Shin, Mitsunaga Fusako, Okada Maki, Shirahama Shuya, Eberle Richard
机构信息
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
出版信息
Clin Diagn Lab Immunol. 2002 May;9(3):716-9. doi: 10.1128/cdli.9.3.716-719.2002.
Abstract
By adding betaine to the PCR mixture, we previously established a PCR method to amplify a DNA segment of the glycoprotein G gene of B virus (BV) derived from a rhesus macaque. We have found that DNA of other BV strains derived from cynomolgus, pigtail, and lion-tailed macaques can also serve as the template in our PCR assay. Under the same conditions no product was obtained with DNA of simian agent 8 of green monkeys and Herpesvirus papio 2 of baboons, or the human herpes simplex viruses types 1 and 2. Thus, this PCR method is useful to discriminate BV from other closely related primate alphaherpesviruses.
摘要
通过在聚合酶链反应（PCR）混合物中添加甜菜碱，我们之前建立了一种PCR方法，用于扩增源自恒河猴的B病毒（BV）糖蛋白G基因的DNA片段。我们发现，源自食蟹猴、猪尾猴和狮尾猕猴的其他BV毒株的DNA也可作为我们PCR检测中的模板。在相同条件下，绿猴的猿猴病毒8和狒狒的狒猴疱疹病毒2的DNA，以及人类单纯疱疹病毒1型和2型，均未获得产物。因此，这种PCR方法有助于区分BV与其他密切相关的灵长类α疱疹病毒。
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