通过聚合酶链反应检测和区分灵长类α疱疹病毒

Detection and differentiation of primate alpha-herpesviruses by PCR.

作者信息

Black D H, Eberle R

机构信息

Department of Veterinary Infectious Diseases and Physiology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078-2006, USA.

出版信息

J Vet Diagn Invest. 1997 Jul;9(3):225-31. doi: 10.1177/104063879700900301.

DOI:10.1177/104063879700900301

PMID:9249159

Abstract

A rapid method for detection and differentiation of 5 primate alpha-herpesviruses (human herpes simplex virus types 1 and 2 [HSV1, HSV2], green monkey simian agent 8, baboon herpesvirus 2 [HVP2], and macaque B virus [BV]) was developed utilizing the polymerase chain reaction (PCR). PCR primers were located in conserved regions of the gene encoding the glycoprotein B, which flanks an intervening region that is highly divergent among the 5 viruses. Amplified PCR products from the 5 viruses were readily differentiated by their unique restriction enzyme digestion patterns. No variation in digestion patterns was noted among strains of HSV1, HSV2, or HVP2. One clinical isolate of BV exhibited variation in a single restriction site, but its overall restriction pattern remained typical of BV. This method (PCR/RFLP) allowed the presence of herpesvirus DNA in clinical swabs from primates to be readily detected and the virus unambiguously identified.

摘要

利用聚合酶链反应（PCR）开发了一种快速检测和区分5种灵长类α疱疹病毒（人类单纯疱疹病毒1型和2型[HSV1、HSV2]、绿猴猿猴因子8、狒狒疱疹病毒2[HVP2]和猕猴B病毒[BV]）的方法。PCR引物位于编码糖蛋白B的基因保守区域，该区域两侧是5种病毒之间高度不同的间隔区域。5种病毒扩增的PCR产物通过其独特的限制性内切酶消化模式很容易区分。HSV1、HSV2或HVP2菌株之间未观察到消化模式的变化。BV的一个临床分离株在单个限制性位点表现出变化，但其总体限制性模式仍为BV的典型模式。这种方法（PCR/限制性片段长度多态性）能够轻松检测灵长类动物临床拭子中疱疹病毒DNA的存在，并明确鉴定病毒。