Yoshinari M, Oda Y, Inoue T, Matsuzaka K, Shimono M
Department of Dental Materials Science, Oral Health Science Center, Tokyo Dental College, Chiba, Japan.
Biomaterials. 2002 Jul;23(14):2879-85. doi: 10.1016/s0142-9612(01)00415-x.
Thin calcium phosphate (Ca-P) coatings have been introduced to overcome the shortcomings of plasma-sprayed Ca-P coatings. In our previous experiments, thin Ca-P coatings also enabled the immobilization of bisphosphonate, which is a drug used to treat osteoporosis. The present study was designed to evaluate the bone response to titanium implants treated with a thin Ca-P coating and bisphosphonate. Forty cylindrical commercially pure titanium implants with a length of 7 mm and a diameter of 3 mm were used as test implant fixtures. Three groups of surface-treated implants were prepared: (1) blasted with titanium powder and etched with a solution of 10% HF + 5% HNO3 (control); (2) modified with 0.5-microm thick Ca-P coatings and rapid heat-treating, and (3) immobilized with bisphosphonate by immersion in pamidronate disodium solution (10(-2) M) for 24 h at 37 degrees C. These surface-treated implants were inserted into edentulous areas in the mandibular molar region of five beagle dogs. After implantation periods of 4 and 12 weeks, the bone implant interface was evaluated histologically and histomorphometrically. All measurements were statistically evaluated using a one-way ANOVA and Fisher PLSD test for multiple comparisons among the means. Four weeks after the implantation, higher percentage of bone contact was found around the thin Ca-P-coated implants compared to that of the control group. The highest percentage of bone contact was found around the bisphosphonate-immobilized implants after 12 weeks of implantation. These data suggest that a thin coating of calcium phosphate followed by bisphosphonate-immobilization is effective in the promotion of osteogenesis on surfaces of dental implants.
薄磷酸钙（Ca-P）涂层已被引入以克服等离子喷涂Ca-P涂层的缺点。在我们之前的实验中，薄Ca-P涂层还能够固定双膦酸盐，这是一种用于治疗骨质疏松症的药物。本研究旨在评估骨对用薄Ca-P涂层和双膦酸盐处理的钛植入物的反应。使用40个长度为7mm、直径为3mm的圆柱形商业纯钛植入物作为测试植入固定装置。制备了三组表面处理的植入物：（1）用钛粉喷砂并用10%HF + 5%HNO3溶液蚀刻（对照组）；（2）用0.5微米厚的Ca-P涂层改性并进行快速热处理，以及（3）通过在37℃下于帕米膦酸钠溶液（10^(-2)M）中浸泡24小时用双膦酸盐固定。将这些表面处理的植入物插入五只比格犬下颌磨牙区域的无牙区。在植入4周和12周后，对骨植入物界面进行组织学和组织形态计量学评估。所有测量值均使用单向方差分析和Fisher PLSD检验进行统计学评估，以对均值进行多重比较。植入后4周，与对照组相比，在薄Ca-P涂层植入物周围发现更高百分比的骨接触。植入12周后，在双膦酸盐固定的植入物周围发现最高百分比的骨接触。这些数据表明，磷酸钙薄涂层随后进行双膦酸盐固定可有效促进牙种植体表面的骨生成。
