Sawaya A C H F, Palma A M, Caetano F M, Marcucci M C, da Silva Cunha I B, Araujo C E P, Shimizu M T
Universidade São Francisco, Centro de Ciências Biológicas e da Saúde, Bragança Paulista, SP, Brazil.
Lett Appl Microbiol. 2002;35(3):203-7. doi: 10.1046/j.1472-765x.2002.01169.x.
Propolis is known for its activity against micro-organisms and different in vitro assays have been used to evaluate this activity, frequently with contradictory results.
Brazilian propolis from the state of São Paulo was extracted by maceration using different concentrations of ethanol and water. The resultant extracts were analysed by chromatographic methods. Several microbiological methods were compared to determine which one best evaluated the activity of the propolis extracts against species of Candida, with average minimal inhibitory concentration values between 6 and 12 mg ml(-1).
Agar dilution in plates showed the clearest results. These were in agreement with the chromatographic analyses, which also identified the active substances.
Although the active substances identified in this sample are typical of Brazilian propolis, their activity against Candida had not been recognized previously, demonstrating the importance of standardizing the correct combination of microbiological and chromatographic analyses.
蜂胶以其抗微生物活性而闻名，已使用不同的体外试验来评估这种活性，结果常常相互矛盾。
采用不同浓度的乙醇和水浸渍法提取巴西圣保罗州的蜂胶。所得提取物用色谱法进行分析。比较了几种微生物学方法，以确定哪种方法最能评估蜂胶提取物对念珠菌属的活性，其平均最低抑菌浓度值在6至12毫克/毫升之间。
平板琼脂稀释法显示的结果最清晰。这些结果与色谱分析结果一致，色谱分析也鉴定出了活性物质。
尽管该样品中鉴定出的活性物质是巴西蜂胶的典型成分，但其对念珠菌的活性此前尚未得到认可，这表明标准化微生物学和色谱分析的正确组合非常重要。
