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本文引用的文献

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Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa.木质部限制作物黄化脉明细菌的培养和血清学检测及其鉴定为木质部难养细菌的一个菌株。
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Causal Role of Xylella fastidiosa in Oleander Leaf Scorch Disease.韧皮部杆菌在夹竹桃叶枯病中的因果作用。
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Fastidious xylem-limited bacterial plant pathogens.苛求的木质部受限细菌植物病原体。
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Insertion-duplication mutagenesis of neisseria: use in characterization of DNA transfer genes in the gonococcal genetic island.淋病奈瑟菌的插入-重复诱变：用于淋球菌基因岛中DNA转移基因的特性分析
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Transposon mutagenesis of Xylella fastidiosa by electroporation of Tn5 synaptic complexes.通过Tn5突触复合体电穿孔对桑黄杆菌进行转座子诱变。
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Stable transformation of the Xylella fastidiosa citrus variegated chlorosis strain with oriC plasmids.用含oriC质粒对木质部难养菌柑橘杂色黄化病菌株进行稳定转化。
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The genome sequence of the plant pathogen Xylella fastidiosa. The Xylella fastidiosa Consortium of the Organization for Nucleotide Sequencing and Analysis.植物病原体桑萎蔫病菌的基因组序列。核苷酸测序与分析组织的桑萎蔫病菌联盟。
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通过同源重组对桑氏假单胞菌柑橘杂色黄化病菌株进行基因破坏。

Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain.

作者信息

Gaurivaud Patrice, Souza Leonardo C A, Virgílio Andrea C D, Mariano Anelise G, Palma Renê R, Monteiro Patrícia B

机构信息

Fundo de Defesa da Citricultura (Fundecitrus), Araraquara, São Paulo, Brazil.

出版信息

Appl Environ Microbiol. 2002 Sep;68(9):4658-65. doi: 10.1128/AEM.68.9.4658-4665.2002.

DOI:10.1128/AEM.68.9.4658-4665.2002
PMID:12200328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC124079/
Abstract

Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.

摘要

通过使用编码β-半乳糖苷酶的bga基因作为模型，评估了在桑黄杆菌中通过同源重组进行的诱变。通过克隆的bga截短拷贝与内源基因之间的同源重组，复制质粒的整合是由一到两个交叉事件产生的，从而导致β-半乳糖苷酶突变体。使用无启动子的氯霉素乙酰转移酶基因来监测靶基因的表达并选择cvaB突变体。