通过同源重组对桑氏假单胞菌柑橘杂色黄化病菌株进行基因破坏。
Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain.
作者信息
Gaurivaud Patrice, Souza Leonardo C A, Virgílio Andrea C D, Mariano Anelise G, Palma Renê R, Monteiro Patrícia B
机构信息
Fundo de Defesa da Citricultura (Fundecitrus), Araraquara, São Paulo, Brazil.
出版信息
Appl Environ Microbiol. 2002 Sep;68(9):4658-65. doi: 10.1128/AEM.68.9.4658-4665.2002.
Abstract
Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.
摘要
通过使用编码β-半乳糖苷酶的bga基因作为模型，评估了在桑黄杆菌中通过同源重组进行的诱变。通过克隆的bga截短拷贝与内源基因之间的同源重组，复制质粒的整合是由一到两个交叉事件产生的，从而导致β-半乳糖苷酶突变体。使用无启动子的氯霉素乙酰转移酶基因来监测靶基因的表达并选择cvaB突变体。
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1
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Curr Microbiol. 1993 Sep;27(3):137-42. doi: 10.1007/BF01576010.
2
Causal Role of Xylella fastidiosa in Oleander Leaf Scorch Disease.韧皮部杆菌在夹竹桃叶枯病中的因果作用。
Phytopathology. 1999 Jan;89(1):53-8. doi: 10.1094/PHYTO.1999.89.1.53.
3
Fastidious xylem-limited bacterial plant pathogens.苛求的木质部受限细菌植物病原体。
Annu Rev Phytopathol. 1996;34:131-51. doi: 10.1146/annurev.phyto.34.1.131.
4
Insertion-duplication mutagenesis of neisseria: use in characterization of DNA transfer genes in the gonococcal genetic island.淋病奈瑟菌的插入-重复诱变：用于淋球菌基因岛中DNA转移基因的特性分析
J Bacteriol. 2001 Aug;183(16):4718-26. doi: 10.1128/JB.183.16.4718-4726.2001.
5
Transposon mutagenesis of Xylella fastidiosa by electroporation of Tn5 synaptic complexes.通过Tn5突触复合体电穿孔对桑黄杆菌进行转座子诱变。
Mol Plant Microbe Interact. 2001 Jun;14(6):701-6. doi: 10.1094/MPMI.2001.14.6.701.
6
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7
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8
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