Martín-Sosa Samuel, Martín María-Jesús, Hueso Pablo
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Spain.
J Nutr. 2002 Oct;132(10):3067-72. doi: 10.1093/jn/131.10.3067.
Milk oligosaccharides can act as soluble receptors that block bacterial adhesion to the different epithelia. Colonization factor antigens (CFA)/I- and CFA/II-expressing enterotoxigenic Escherichia coli (ETEC) strains constitute one of the main causes of diarrhea in infants. Here, the inhibition of hemagglutination mediated by these strains by milk oligosaccharides was tested. Human milk oligosaccharides showed a strong inhibitory capacity, which decreased when the oligosaccharides were desialylated. Because milk oligosaccharides also are present in the urine of neonates receiving mothers' milk, their ability to bind two uropathogenic Escherichia coli (UPEC) strains was also examined. UPEC strains expressing P (Pap) and P-like (Prs) fimbriae are responsible for infections of the urinary tract such as pyelonephritis and cystitis. The hemagglutination mediated by these strains was inhibited by human milk oligosaccharides. The sialylated fraction was partially responsible for this inhibition in the case of the UPEC expressing the P-like fimbria because differences were found after desialylation. Although bovine milk oligosaccharides were less efficient at inhibiting the hemagglutination of ETEC strains, they were still quite good inhibitors of UPEC strains.
乳寡糖可作为可溶性受体，阻止细菌黏附于不同上皮细胞。表达定居因子抗原（CFA）/I和CFA/II的产肠毒素大肠杆菌（ETEC）菌株是婴儿腹泻的主要病因之一。在此，测试了乳寡糖对这些菌株介导的血凝反应的抑制作用。人乳寡糖表现出很强的抑制能力，去唾液酸化后这种能力会降低。由于接受母乳的新生儿尿液中也存在乳寡糖，因此还检测了它们结合两种尿路致病性大肠杆菌（UPEC）菌株的能力。表达P菌毛（Pap）和P样菌毛（Prs）的UPEC菌株可导致肾盂肾炎和膀胱炎等泌尿系统感染。人乳寡糖可抑制这些菌株介导的血凝反应。对于表达P样菌毛的UPEC菌株，唾液酸化部分对这种抑制作用有一定贡献，因为去唾液酸化后发现了差异。尽管牛乳寡糖在抑制ETEC菌株血凝反应方面效率较低，但它们仍是UPEC菌株的良好抑制剂。
