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2
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Construction and characterization of mutants of the TEM-1 beta-lactamase containing amino acid substitutions associated with both extended-spectrum resistance and resistance to beta-lactamase inhibitors.构建并鉴定含有与超广谱耐药性和对β-内酰胺酶抑制剂耐药性相关氨基酸取代的TEM-1β-内酰胺酶突变体。
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In vitro selection and characterization of mutants in TEM-1-producing Escherichia coli by ceftazidime and ceftibuten.通过头孢他啶和头孢布烯对产TEM-1型大肠杆菌突变体进行体外筛选与鉴定
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A new TEM-derived extended-spectrum beta-lactamase (TEM-91) with an R164C substitution at the omega-loop confers ceftazidime resistance.一种新的源自透射电子显微镜（TEM）的超广谱β-内酰胺酶（TEM-91），其ω-环处发生R164C取代，赋予了对头孢他啶的耐药性。
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本文引用的文献

1
Characterization of a new extended-spectrum beta-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey.在一项意大利调查中，奇异变形杆菌分离出的一种新型超广谱β-内酰胺酶（TEM-87）的特性分析。
Antimicrob Agents Chemother. 2002 Mar;46(3):925-8. doi: 10.1128/AAC.46.3.925-928.2002.
2
Molecular characterization of extended-spectrum beta-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey.一项意大利全国性调查中分离出的医院内肠杆菌科菌株所产超广谱β-内酰胺酶的分子特征分析
J Clin Microbiol. 2002 Feb;40(2):611-4. doi: 10.1128/JCM.40.2.611-614.2002.
3
Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat.21世纪的超广谱β-内酰胺酶：这种重要耐药威胁的特征、流行病学及检测
Clin Microbiol Rev. 2001 Oct;14(4):933-51, table of contents. doi: 10.1128/CMR.14.4.933-951.2001.
4
New TEM variant (TEM-92) produced by Proteus mirabilis and Providencia stuartii isolates.奇异变形杆菌和斯氏普罗威登斯菌分离株产生的新型 TEM 变体（TEM-92）。
Antimicrob Agents Chemother. 2001 Apr;45(4):1278-80. doi: 10.1128/AAC.45.4.1278-1280.2001.
5
Extended-spectrum TEM- and SHV-type beta-lactamase-producing Klebsiella pneumoniae strains causing outbreaks in intensive care units in Italy.在意大利重症监护病房引发疫情的产超广谱 TEM 型和 SHV 型β-内酰胺酶的肺炎克雷伯菌菌株。
Eur J Clin Microbiol Infect Dis. 2000 Oct;19(10):765-72. doi: 10.1007/s100960000363.
6
A secondary drug resistance mutation of TEM-1 beta-lactamase that suppresses misfolding and aggregation.一种抑制错误折叠和聚集的TEM-1β-内酰胺酶的继发性耐药突变。
Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):283-8. doi: 10.1073/pnas.98.1.283.
7
Survey of extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae: prevalence of TEM-52 in Korea.大肠埃希菌和肺炎克雷伯菌临床分离株中超广谱β-内酰胺酶的调查：韩国TEM-52的流行情况
J Clin Microbiol. 1999 Jun;37(6):1758-63. doi: 10.1128/JCM.37.6.1758-1763.1999.
8
A novel extended-spectrum TEM-type beta-lactamase (TEM-52) associated with decreased susceptibility to moxalactam in Klebsiella pneumoniae.一种新型超广谱TEM型β-内酰胺酶（TEM-52），与肺炎克雷伯菌对羟羧氧酰胺菌素敏感性降低有关。
Antimicrob Agents Chemother. 1998 Jan;42(1):108-13. doi: 10.1128/AAC.42.1.108.
9
Extended-spectrum beta-lactamases and other enzymes providing resistance to oxyimino-beta-lactams.超广谱β-内酰胺酶及其他赋予对氧亚氨基β-内酰胺耐药性的酶
Infect Dis Clin North Am. 1997 Dec;11(4):875-87. doi: 10.1016/s0891-5520(05)70395-0.
10
Characterization of a new TEM-derived beta-lactamase produced in a Serratia marcescens strain.粘质沙雷氏菌菌株中产生的一种新型透射电子显微镜衍生β-内酰胺酶的特性分析
Antimicrob Agents Chemother. 1997 Nov;41(11):2374-82. doi: 10.1128/AAC.41.11.2374.

TEM-92超广谱β-内酰胺酶的生化特性，一种信号肽不同于TEM-52的蛋白质。

Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the signal peptide.

作者信息

Perilli Mariagrazia, Segatore Bernardetta, De Massis Maria Rosaria, Pagani Laura, Luzzaro Francesco, Rossolini Gian Maria, Amicosante Gianfranco

机构信息

Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, Italy.

出版信息

Antimicrob Agents Chemother. 2002 Dec;46(12):3981-3. doi: 10.1128/AAC.46.12.3981-3983.2002.

DOI:10.1128/AAC.46.12.3981-3983.2002
PMID:12435705
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC132775/
Abstract

A bla(TEM-92) gene was cloned from a Proteus mirabilis isolate and expressed in Escherichia coli. Production of the enzyme caused reduction of susceptibility to penicillins and narrow- to expanded-spectrum cephalosporins but not to moxalactam and cephamycins. Determination of kinetic parameters with the purified enzyme revealed hydrolysis of expanded-spectrum cephalosporins, while cephamycins, moxalactam, and aztreonam were very poorly or not hydrolyzed. Clavulanate and penicillanic acid sulfones acylated TEM-92 slowly, and deacylation occurred at measurable rates.

摘要

从一株奇异变形杆菌分离株中克隆出bla(TEM-92)基因，并在大肠杆菌中表达。该酶的产生导致对青霉素和窄谱至广谱头孢菌素的敏感性降低，但对莫西沙星和头霉素不敏感。用纯化的酶测定动力学参数表明，广谱头孢菌素可被水解，而头霉素、莫西沙星和氨曲南很少被水解或不被水解。克拉维酸和青霉素烷砜对TEM-92的酰化作用缓慢，且脱酰化以可测量的速率发生。