Ho Joseph X, Ruble John R, McInnis Thomas R, Carter Daniel C, Huang Huapeng, Gibson Walter M
New Century Pharmaceuticals Inc, 895 Martin Road, Huntsville, AL 35824, USA.
Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2087-95. doi: 10.1107/s0907444902017511. Epub 2002 Nov 23.
A data-collection method for macromolecular crystals using convergent sources is described here. Because of the unique characteristics of the diffraction patterns, a software package CBMPRO has been developed specifically for processing data images collected with the convergent beam method (CBM). The resulting data sets from crystals with two different sets of unit-cell parameters are presented and compared. There is good agreement between data sets from the same type of crystals under slightly different experimental conditions and data sets collected and processed with CBM also agree well with those from conventional oscillation methods, marking an important step to establishing CBM as a viable alternate data-collection method for macromolecular crystals.
本文描述了一种使用会聚光源收集大分子晶体数据的方法。由于衍射图案的独特特性，专门开发了一个软件包CBMPRO，用于处理用会聚束方法（CBM）收集的数据图像。展示并比较了来自具有两组不同晶胞参数的晶体的数据集。在略有不同的实验条件下，来自同一类型晶体的数据集之间具有良好的一致性，并且用CBM收集和处理的数据集与来自传统振荡方法的数据集也吻合得很好，这标志着将CBM确立为大分子晶体可行的替代数据收集方法的重要一步。
