坏死性视网膜炎房水样本的聚合酶链反应分析
Polymerase chain reaction analysis of aqueous humour samples in necrotising retinitis.
作者信息
Tran T H C, Rozenberg F, Cassoux N, Rao N A, LeHoang P, Bodaghi B
机构信息
Department of Ophthalmology, Pitié-Salpêtriére Hospital, Paris, France.
出版信息
Br J Ophthalmol. 2003 Jan;87(1):79-83. doi: 10.1136/bjo.87.1.79.
AIM
To evaluate the diagnostic value of polymerase chain reaction (PCR) performed on aqueous humour for the detection of viral DNA in patients with necrotising herpetic retinitis.
METHODS
The clinical features and laboratory results of 22 patients (29 eyes) presenting with necrotising herpetic retinitis between March 1999 and June 2001 were reviewed retrospectively. Aqueous humour was obtained after anterior chamber paracentesis and PCR was performed in all cases.
RESULTS
Viral DNA was detected in the aqueous humour of 19 patients (86.4%). Epstein-Barr virus (EBV) seroconversion was evidenced in one additional patient. In the acute retinal necrosis (ARN) group (n = 19), varicella zoster virus (VZV) DNA was identified in six patients, herpes simplex virus 1 (HSV-1) DNA in two patients, herpes simplex virus 2 (HSV-2) DNA in four patients, and cytomegalovirus (CMV) genome in four patients. In the progressive outer retinal necrosis (PORN) group (n = 3), VZV DNA was detected in all patients. No sample was positive for more than one virus.
CONCLUSIONS
PCR analysis of aqueous humour in patients with clinical features of necrotising viral retinitis can provide specific aetiological orientation and the method appears to be safe and highly sensitive.
目的
评估对坏死性疱疹性视网膜炎患者房水进行聚合酶链反应（PCR）检测病毒DNA的诊断价值。
方法
回顾性分析1999年3月至2001年6月间22例（29只眼）坏死性疱疹性视网膜炎患者的临床特征和实验室检查结果。所有病例均在前房穿刺术后获取房水并进行PCR检测。
结果
19例患者（86.4%）的房水中检测到病毒DNA。另有1例患者出现EB病毒（EBV）血清学转换。在急性视网膜坏死（ARN）组（n = 19）中，6例患者检测到水痘带状疱疹病毒（VZV）DNA，2例患者检测到单纯疱疹病毒1型（HSV-1）DNA，4例患者检测到单纯疱疹病毒2型（HSV-2）DNA，4例患者检测到巨细胞病毒（CMV）基因组。在进行性外层视网膜坏死（PORN）组（n = 3）中，所有患者均检测到VZV DNA。没有样本对一种以上病毒呈阳性。
结论
对具有坏死性病毒性视网膜炎临床特征的患者进行房水PCR分析可提供特定的病因学方向，且该方法似乎安全且高度敏感。
Zotero 插件