Kakizoe T, Takayasu H, Kawachi T, Sugimura T, Takeuchi T
J Natl Cancer Inst. 1976 Feb;56(2):433-5. doi: 10.1093/jnci/56.2.433.
Leupeptin, isolated from Actinomycetes, is a potent and specific inhibitor of proteases. We found that the administration of leupeptin enhanced that size of urinary bladder tumors induced in rats by the oral administration of N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). BBN was given as a 0.05% solution in the drinking water for 6 weeks, and then animals were fed a diet with or without 0.1% leupeptin for 30 weeks. The average weight of the bladders with tumors in rats fed a leupeptin diet was about eight times that of rats on a diet without leupeptin, though the incidences and average numbers of tumors in the bladders were similar in the two groups.
亮抑蛋白酶肽是从放线菌中分离出来的一种强效且特异性的蛋白酶抑制剂。我们发现，给予亮抑蛋白酶肽会使经口服N-丁基-N-(4-羟丁基)亚硝胺（BBN）诱导的大鼠膀胱肿瘤体积增大。BBN以0.05%的溶液形式添加到饮用水中，持续6周，然后给动物喂食含或不含0.1%亮抑蛋白酶肽的饲料，持续30周。食用含亮抑蛋白酶肽饲料的大鼠中带有肿瘤的膀胱平均重量约为食用不含亮抑蛋白酶肽饲料大鼠的八倍，尽管两组膀胱肿瘤的发生率和平均数量相似。
