Giakkoupi P, Petrikkos G, Tzouvelekis L S, Tsonas S, Legakis N J, Vatopoulos A C
Department of Hygiene & Epidemiology, Medical School, Athens University, Greece.
J Clin Microbiol. 2003 Feb;41(2):822-5. doi: 10.1128/JCM.41.2.822-825.2003.
Fifty-eight imipenem-nonsusceptible (MIC >or= 8 microg/ml) Pseudomonas aeruginosa strains isolated during May 2001 in 15 Greek hospitals were studied. Thirty-six isolates derived from nine hospitals carried VIM-type metallo-beta-lactamase genes, as found by PCR. In 34 isolates, bla(VIM) was associated with class 1 integrons of various sizes. DNA sequencing indicated the presence of bla(VIM-2) gene cassettes in a variety of integron structures. Random amplified polymorphic DNA typing suggested diversity of the bla(VIM)-positive strains. Synergy between 2-mercaptoacetic acid and imipenem indicated carbapenemase activity in 26 bla(VIM)-positive strains.
对2001年5月在希腊15家医院分离出的58株对亚胺培南不敏感(MIC≥8μg/ml)的铜绿假单胞菌菌株进行了研究。通过PCR发现,来自9家医院的36株分离菌携带VIM型金属β-内酰胺酶基因。在34株分离菌中,bla(VIM)与各种大小的1类整合子相关。DNA测序表明在多种整合子结构中存在bla(VIM-2)基因盒。随机扩增多态性DNA分型表明bla(VIM)阳性菌株具有多样性。2-巯基乙酸与亚胺培南之间的协同作用表明26株bla(VIM)阳性菌株具有碳青霉烯酶活性。