Cornaglia G, Mazzariol A, Lauretti L, Rossolini G M, Fontana R
1Istituto di Microbiologia, Università di Verona, Verona, Italy.
Clin Infect Dis. 2000 Nov;31(5):1119-25. doi: 10.1086/317448. Epub 2000 Nov 6.
A total of 8 Pseudomonas aeruginosa isolates was collected from 7 different patients in different wards of the University Hospital of Verona, Italy, from February 1997 to February 1998. The high level of resistance to carbapenems (imipenem minimum inhibitory concentration was always >128 microg/mL) and other broad-spectrum beta-lactams and the rate of imipenem hydrolysis and its inhibition by ethylenediamine-tetra-acetic acid were all suggestive of production of a carbapenem-hydrolyzing metallo-beta-lactamase. A specific DNA probe derived from the recently cloned bla(VIM-1) gene hybridized to all the isolates. A genomic DNA fingerprinting profile revealed clonal relatedness for 7 of 8 isolates. A description of this hospital outbreak is reported, the occurrence of which confirms that proliferation of metallo-beta-lactamase-producing strains multiply resistant to beta-lactams is already a reality outside Japan. These findings emphasize the need for early recognition of similar isolates.
1997年2月至1998年2月期间，从意大利维罗纳大学医院不同病房的7名不同患者中总共收集了8株铜绿假单胞菌分离株。这些分离株对碳青霉烯类药物（亚胺培南最低抑菌浓度始终>128μg/mL）以及其他广谱β-内酰胺类药物具有高度耐药性，且亚胺培南水解率及其受乙二胺四乙酸抑制的情况均提示产生了碳青霉烯水解金属β-内酰胺酶。源自最近克隆的bla(VIM-1)基因的特异性DNA探针与所有分离株杂交。基因组DNA指纹图谱显示8株分离株中有7株具有克隆相关性。本文报道了此次医院暴发情况，其发生证实了产金属β-内酰胺酶且对β-内酰胺类药物多重耐药的菌株的增殖在日本境外已成为现实。这些发现强调了早期识别类似分离株的必要性。
