Nowak Jerzy, Januszkiewicz Danuta, Pernak Monika, Liweń Izabela, Zawada Mariola, Rembowska Jolanta, Nowicka Karina, Lewandowski Krzysztof, Hertmanowska Hanna, Wender Mieczyslaw
Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska, Poland.
J Neurovirol. 2003 Feb;9(1):112-7. doi: 10.1080/13550280390173355.
In the etiopathogenesis of multiple sclerosis (MS), both genetic and environmental factors play an important role. Among environmental factors, viral infections are most likely connected with the etiology of MS. There are many evidence suggesting possible involvement of retroviruses in the development of autoimmune diseases including MS. Multiple sclerosis-associated retrovirus (MSRV) seems to be the important candidate for viral etiology of MS. The aim of the study was to analyze MSRV pol sequences in patients with MS. As control, groups of myasthenia gravis, Parkinson's disease, and migraine patients, and healthy individuals have been studied. The MSRV pol sequences have been analyzed in RNA isolated from the serum and in DNA and RNA of peripheral blood lymphocytes from untreated MS patients and control groups. The MSRV pol sequences have been detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and PCR technique, using specific oligonucleotide primers. In the serum RNA (cDNA), MSRV pol sequences have been identified in 31/32 MS patients. MSRV pol sequences were detected in serum cDNA of 9/17 myasthenia gravis patients, 7/16 Parkinson's disease patients, 10/21 migraine patients, and 13/27 healthy individuals. MSRV pol sequences were observed also in RNA from lymphocytes of all MS patients, 12/17 myasthenia gravis patients, 9/16 Parkinson's disease patients, 14/21 migraine patients, and 18/27 healthy donors. In the DNA from peripheral blood lymphocytes of all studied patients and healthy individuals, MSRV pol sequences have been found. The observed pattern of fiber-fluorescence in situ hybridization (FISH) signals suggests the presence of multiple copies of MSRV pol sequences, most likely tandemly dispersed in the genome. It can be concluded that MSRV pol sequences are endogenous, widespread in lymphocytes DNA, and transcribed into RNA of MS patients as well as of other studied patients and healthy individuals. However, more frequent expression of MSRV sequences detected in lymphocytes RNA (cDNA), as well as their presence in higher frequency in the serum of MS patients, may suggest the involvement of MSRV in the etiopathogenesis on MS.
在多发性硬化症（MS）的病因发病机制中，遗传因素和环境因素均发挥着重要作用。在环境因素中，病毒感染极有可能与MS的病因相关。有诸多证据表明逆转录病毒可能参与包括MS在内的自身免疫性疾病的发展。多发性硬化症相关逆转录病毒（MSRV）似乎是MS病毒病因的重要候选者。本研究的目的是分析MS患者的MSRV pol序列。作为对照，对重症肌无力患者、帕金森病患者、偏头痛患者群体以及健康个体进行了研究。已对未经治疗的MS患者和对照组血清中分离出的RNA以及外周血淋巴细胞的DNA和RNA中的MSRV pol序列进行了分析。采用特异性寡核苷酸引物，通过逆转录聚合酶链反应（RT-PCR）和PCR技术检测MSRV pol序列。在血清RNA（cDNA）中，31/32例MS患者中鉴定出了MSRV pol序列。在9/17例重症肌无力患者、7/16例帕金森病患者、10/21例偏头痛患者以及13/27例健康个体的血清cDNA中检测到了MSRV pol序列。在所有MS患者、12/17例重症肌无力患者、9/16例帕金森病患者、14/21例偏头痛患者以及18/27例健康供体的淋巴细胞RNA中也观察到了MSRV pol序列。在所有研究患者和健康个体的外周血淋巴细胞DNA中均发现了MSRV pol序列。观察到的纤维荧光原位杂交（FISH）信号模式表明存在多个MSRV pol序列拷贝，很可能串联分散在基因组中。可以得出结论，MSRV pol序列是内源性的，广泛存在于淋巴细胞DNA中，并转录为MS患者以及其他研究患者和健康个体的RNA。然而，在淋巴细胞RNA（cDNA）中检测到的MSRV序列更频繁的表达，以及它们在MS患者血清中更高的频率存在，可能表明MSRV参与了MS的病因发病机制。
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