Myhre B A, Walker L J, White M L
Department of Pathology, U.C.L.A. School of Medicine, Los Angeles, California, USA.
Transfusion. 1974 Mar-Apr;14(2):116-23. doi: 10.1111/j.1537-2995.1974.tb04502.x.
Platelet concentrates were contaminated with predetermined numbers of bacteria isolated from patients. Staphylococcus epidermidis and Pseudomonas aeruginosa organisms were killed if the number introduced was less than 10(3). When 10(4) through 10(6) organisms were added, their growth was depressed for 24 hours and then slowly resumed. If more than 10(7) organisms were used, a slow and progressive growth occurred. After the concentrates were contaminated with Staphylocoecus aureus, slow progressive growth occurred regardless of the size of the inoculum. Cultures taken from sample phlebotomies performed on a few donors showed that the number and type of organisms released into the blood during the venepuncture are such that they could usually, but not always, be inactivated by the bacteriocidal property of the platelet packs. This bacteriocidal property will usually allow the platelet packs to be stored at room temperature for considerable amounts of time with relative safety. Bacterial growth will normally occur only when the number of organisms introduced during the venepuncture is quite large, and growth will usually occur during the first 24 hours.
血小板浓缩物被预先确定数量的从患者身上分离出的细菌污染。如果引入的表皮葡萄球菌和铜绿假单胞菌数量少于10³，这些微生物会被杀死。当加入10⁴至10⁶个微生物时，它们的生长会被抑制24小时，然后缓慢恢复。如果使用超过10⁷个微生物，就会出现缓慢且持续的生长。血小板浓缩物被金黄色葡萄球菌污染后，无论接种量大小，都会出现缓慢的持续生长。对一些献血者进行的样本静脉穿刺采集的培养显示，静脉穿刺过程中释放到血液中的微生物数量和类型通常使得它们能够被血小板包的杀菌特性灭活，但并非总是如此。这种杀菌特性通常会使血小板包在相对安全的情况下在室温下储存相当长的时间。细菌通常仅在静脉穿刺时引入的微生物数量相当大时才会生长，且生长通常会在最初24小时内发生。
