Morita Eiko, Narikiyo Michihiro, Yano Akira, Nishimura Eisaku, Igaki Hiroyasu, Sasaki Hiroki, Terada Masaaki, Hanada Nobuhiro, Kawabe Ryoichi
Department of Oral Health, National Institute of Public Health, Shinjuku-ku, Tokyo 162-8640, Japan.
Cancer Sci. 2003 Jun;94(6):492-6. doi: 10.1111/j.1349-7006.2003.tb01471.x.
Multiple cancers frequently occur in the upper aerodigestive tract. The high incidence rate of multiple carcinomas in this region is often explained in terms of involvement of the same underlying risk factors. It has been reported that the oral bacterium Streptococcus anginosus (S. anginosus) is associated with esophageal, gastric, and pharyngeal cancer tissues. In this study, a highly specific quantification method for S. anginosus DNA using real-time PCR was established. We employed this assay to determine whether S. anginosus is also associated with oral cancer tissues. This precise quantification method revealed different degrees of infection with S. anginosus in esophageal cancer and oral cancer. We assayed 10 ng of genomic DNA from cancer tissues, and found that eight of 18 samples (44%) from the esophagus contained a detectable level (>10 fg) of S. anginosus DNA, whereas this was the case for only five of 38 samples (13%) of oral cancer. The quantity of S. anginosus DNA in the esophageal cancer tissues was significantly higher than in oral cancer. The maximum amount of S. anginosus DNA was approximately ten times higher in esophageal than in oral cancer tissues. In addition, none of the five different oral cancer sites (floor of the mouth, mandibular gingival, maxillary gingival, buccal mucosal, and tongue) showed significant signs of S. anginosus infection. On the other hand, most non-cancerous tissues of the esophagus and tongue showed an undetectable level of S. anginosus. These results suggest that S. anginosus is associated with esophageal cancer, but is not closely related with oral cancer.
多种癌症常发生于上消化道。该区域多原发癌的高发病率常根据相同潜在危险因素的影响来解释。据报道，口腔细菌咽峡炎链球菌（S. anginosus）与食管癌、胃癌和咽喉癌组织有关。在本研究中，建立了一种使用实时PCR对咽峡炎链球菌DNA进行高特异性定量的方法。我们采用该检测方法来确定咽峡炎链球菌是否也与口腔癌组织有关。这种精确的定量方法揭示了食管癌和口腔癌中咽峡炎链球菌的不同感染程度。我们检测了来自癌组织的10 ng基因组DNA，发现18份食管样本中有8份（44%）含有可检测水平（>10 fg）的咽峡炎链球菌DNA，而在38份口腔癌样本中只有5份（13%）是这种情况。食管癌组织中咽峡炎链球菌DNA的含量显著高于口腔癌。食管癌组织中咽峡炎链球菌DNA的最大含量比口腔癌中约高10倍。此外，五个不同的口腔癌部位（口底、下颌牙龈、上颌牙龈、颊黏膜和舌）均未显示出明显的咽峡炎链球菌感染迹象。另一方面，食管和舌的大多数非癌组织显示咽峡炎链球菌水平不可检测。这些结果表明，咽峡炎链球菌与食管癌有关，但与口腔癌关系不密切。
