Williams Josie, Clarkson John M, Mills Peter R, Cooper Richard M
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom.
Appl Environ Microbiol. 2003 Jul;69(7):4190-1. doi: 10.1128/AEM.69.7.4190-4191.2003.
We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.
我们改良了一种先前开发用于从土壤中重新分离木霉属菌株的选择性培养基，用于定量测定哈茨木霉在商业双孢蘑菇堆肥中的生长情况。这种培养基能够比较具有侵袭性的（即对双孢蘑菇产量有抑制作用的）哈茨木霉群体和无侵袭性的群体。所得培养基含有抗菌剂氯霉素、链霉素、五氯硝基苯和霜霉威，具有高度选择性，能够以紧凑菌落的形式回收哈茨木霉，以活分生孢子和菌丝片段的形式存在，且无可见的微生物污染物。
