Zehnder Matthias, Delaleu Nicolas, Du Yunling, Bickel Matthias
Division of Endodontics, School of Dental and Oral Surgery, Columbia University, New York, NY, USA.
Cytokine. 2003 May;22(3-4):84-8. doi: 10.1016/s1043-4666(03)00116-9.
Analyses of cytokines mediating inflammatory reactions are key to understanding the etiopathology of various diseases. This study investigated differences in cytokine gene expression between pulps from healthy virgin teeth and from symptomatic vital teeth with severe caries lesions in a group of young, healthy individuals. The mRNA levels of IL-1alpha, IL-1beta, IL-6, IL-8, and IL-18 were measured concomitantly by quantitative real-time RT-PCR. IL-1alpha and IL-1beta were not expressed at significantly higher levels in symptomatic versus clinically healthy pulps, while the difference was significant for the other cytokines (log-rank test, P<0.05). A concordance test for independence revealed significant correlation between IL-1alpha and IL-1beta, and between IL-6, IL-8, and IL-18 mRNA levels (P<0.05). The cytokine-specific differences revealed a differential significance of gene expression in cytokine regulation. The hypothesis that increase of cytokine mRNA expression is part of host reaction in pulpitis was corroborated by our observation.
对介导炎症反应的细胞因子进行分析是理解各种疾病病因病理学的关键。本研究调查了一组年轻健康个体中，健康恒牙牙髓与患有严重龋损的有症状活髓牙牙髓之间细胞因子基因表达的差异。通过定量实时逆转录聚合酶链反应同步测量白细胞介素-1α（IL-1α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-18（IL-18）的信使核糖核酸（mRNA）水平。与临床健康牙髓相比，有症状牙髓中IL-1α和IL-1β的表达水平没有显著升高，而其他细胞因子的差异具有统计学意义（对数秩检验，P<0.05）。独立性一致性检验显示IL-1α和IL-1β之间，以及IL-6、IL-8和IL-18 mRNA水平之间存在显著相关性（P<0.05）。细胞因子特异性差异揭示了细胞因子调节中基因表达的不同意义。我们的观察结果证实了细胞因子mRNA表达增加是牙髓炎宿主反应一部分的假设。
