Shiwaku K, Nogi A, Anuurad E, Kitajima K, Enkhmaa B, Shimono K, Yamane Y
Department of Environmental Medicine, Shimane Medical, University, Izumo City, Shimane, Japan.
Int J Obes Relat Metab Disord. 2003 Sep;27(9):1028-36. doi: 10.1038/sj.ijo.0802375.
Trp64Arg mutation in the beta(3)-adrenergic receptor (beta(3)AR) gene is relatively common in Japanese people. However, it has not been clear whether persons with Trp64Arg mutation in the beta(3)AR gene tend to have obesity and difficulty in losing weight even with a restricted diet and exercise. We investigated the response of body weight and metabolic factors to behavioral intervention in Japanese women with Trp64Arg mutation in the beta(3)AR gene.
A 3-month behavioral intervention study using a combination of diet and exercise programs.
A total of 76 perimenopausal women with no clinical symptoms (age: 54.7+/-7.7 y, body mass index (BMI): 21.0-33.0 kg/m(2)).
Anthropometric measurements (weight, height, body fat, waist circumference, hip circumference, skin fold, resting energy expenditure and blood pressure) and metabolic measurements (serum levels of cholesterol, triglyceride, phospholipid, nonesterified fatty acid, glucose, insulin and leptin) and determination of the beta(3)AR genotype by polymerase chain reaction followed by BstNI digestion.
At the baseline of BMI, body weight, body fat, waist circumference, hip circumference, the arm skin fold, resting energy expenditure, or blood lipid and glucose profiles, there was no significant difference in participants with/without mutation of the beta(3)AR gene. The intervention yielded a body weight reduction in 69 and 48%, and induced a significant difference in weight loss (-0.74 and -0.01 kg) for women with wild-type and Trp64Arg mutation, respectively. Significant differences of anthropometric parameters were found in body weight, BMI, waist and hip circumferences and blood pressure of wild type by the intervention. However, women with Trp64Arg mutation did not show significant changes in these anthropometric parameters, except for hip circumference. A significant difference was found in high-density lipoprotein cholesterol (HDL-C) and in the low-density lipoprotein cholesterol/HDL-C ratio in both genotypes.
The results of the present study suggest that the Trp64Arg mutation of the beta(3)AR gene is associated with difficulty in losing weight through behavioral intervention, although it is not related to obesity-related phenotypes and resting energy expenditure before the intervention.
β（3）-肾上腺素能受体（β（3）AR）基因中的色氨酸64精氨酸（Trp64Arg）突变在日本人中较为常见。然而，β（3）AR基因发生Trp64Arg突变的人是否更容易肥胖，以及即使通过限制饮食和运动也难以减肥，目前尚不清楚。我们研究了β（3）AR基因发生Trp64Arg突变的日本女性体重和代谢因素对行为干预的反应。
一项为期3个月的行为干预研究，采用饮食和运动计划相结合的方式。
共有76名无临床症状的围绝经期女性（年龄：54.7±7.7岁，体重指数（BMI）：21.0 - 33.0 kg/m²）。
人体测量指标（体重、身高、体脂、腰围、臀围、皮褶厚度、静息能量消耗和血压）、代谢指标（血清胆固醇、甘油三酯、磷脂、非酯化脂肪酸、葡萄糖、胰岛素和瘦素水平），以及通过聚合酶链反应继以BstNI酶切来测定β（3）AR基因型。
在BMI、体重、体脂、腰围、臀围、上臂皮褶厚度、静息能量消耗或血脂及血糖谱的基线水平上，β（3）AR基因有无突变的参与者之间无显著差异。干预使69%和48%的参与者体重减轻，野生型和Trp64Arg突变型女性的体重减轻量分别有显著差异（-0.74和-0.01 kg）。干预后，野生型在体重、BMI、腰围和臀围以及血压等人体测量参数上有显著差异。然而，除臀围外，Trp64Arg突变型女性在这些人体测量参数上未显示出显著变化。两种基因型在高密度脂蛋白胆固醇（HDL-C）以及低密度脂蛋白胆固醇/HDL-C比值上均存在显著差异。
本研究结果表明，β（3）AR基因的Trp64Arg突变与通过行为干预减肥困难有关，尽管它与干预前的肥胖相关表型和静息能量消耗无关。
