Rodríguez-Mercado Juan José, Roldán-Reyes Elia, Altamirano-Lozano Mario
Unidad de Investigación en Genética y Toxicología Ambiental, Bioterio Campo-II, Facultad de Estudios Superiores-Zaragoza, UNAM A.P. 9-020, Mexico 15000, D.F., Mexico.
Toxicol Lett. 2003 Oct 15;144(3):359-69. doi: 10.1016/s0378-4274(03)00255-8.
Vanadium has been considered an aneuploidogen; however, there is controversial information about the clastogenic effects of vanadium compounds. In this study, the genotoxicity of vanadium(IV) tetraoxide (V(2)O(4)) was evaluated in human cultured lymphocytes and leukocytes using the mitotic index (MI), the replicative index (RI), chromosome aberrations (CA), sister chromatid exchanges (SCE), satellite associations (SA) and the single cell gel electrophoresis (SCGE) assay. This chemical induced a clear dose-response in MI inhibitions and modifications in the RI. In the CA, including breaks and exchanges and in the SCE, a significant increase appeared in the treated group compared with the controls. The SA test did not reveal an important difference. For the detection of genotoxic properties of vanadium(IV) using the SCGE assay, the 2 h evaluation period was not long enough for the chemical to enter the cell. These results indicate that vanadium(IV) tetraoxide is capable of inducing cytotoxic and cytostatic effects and chromosomal damage.
钒被认为是一种非整倍体诱变剂；然而，关于钒化合物的致断裂效应存在有争议的信息。在本研究中，使用有丝分裂指数（MI）、复制指数（RI）、染色体畸变（CA）、姐妹染色单体交换（SCE）、卫星联合（SA）和单细胞凝胶电泳（SCGE）试验，评估了四氧化钒（V(2)O(4)）对人培养淋巴细胞和白细胞的遗传毒性。这种化学物质在MI抑制和RI改变方面诱导了明显的剂量反应。在包括断裂和交换的CA以及SCE中，与对照组相比，处理组出现了显著增加。SA试验未显示出重要差异。对于使用SCGE试验检测钒（IV）的遗传毒性特性，2小时的评估期不足以使该化学物质进入细胞。这些结果表明，四氧化钒能够诱导细胞毒性和细胞生长抑制效应以及染色体损伤。
