Gallinella Giorgio, Venturoli Simona, Manaresi Elisabetta, Musiani Monica, Zerbini Marialuisa
Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Via Massarenti, 9, I-40138 Bologna, Italy.
J Clin Virol. 2003 Sep;28(1):1-13. doi: 10.1016/s1386-6532(03)00120-3.
Genetic analysis of parvovirus B19 has been carried out mainly to establish a framework to track molecular epidemiology of the virus and to correlate sequence variability with different pathological and clinical manifestations of the virus. A good amount of information regarding B19 virus sequence variability is available, and presently there are about 400 sequence records deposited in the nucleotide database of NCBI. A few are almost complete genomic sequences, and these allow the construction of a global alignment framework. Many others are partial genomic sequences, limited to selected regions, and these allow comparison of a higher number of isolates from well-defined epidemiological settings and/or pathological conditions. Most studies showed that the genetic variability of B19 virus is low, that molecular epidemiology is possible only on a limited geographical and temporal setting, and that no clear correlations are present between genome sequence and distinctive pathological and clinical manifestations. More recently, several viral isolates have been identified that show remarkable sequence diversity with respect to reference sequences. The identification of variant isolates added to the knowledge of genetic diversity in this virus group and allowed the identification of three divergent genetic clusters, about 10% divergent from each other and still quite distinct from other parvoviruses, that can be thought of as different genotypes within the human erythrovirus group and that show clearly resolved phylogenetic relationship. These variant isolates pose interesting questions regarding the real extent of genetic variability in the human erythroviruses, the relevance of these viruses in terms of epidemiology and their possible implication in the pathogenesis of erythrovirus-related diseases.
对细小病毒B19进行基因分析，主要是为了建立一个框架，以追踪该病毒的分子流行病学，并将序列变异性与该病毒的不同病理和临床表现相关联。目前已有大量关于B19病毒序列变异性的信息，NCBI的核苷酸数据库中目前已存有约400条序列记录。其中一些几乎是完整的基因组序列，这些序列可用于构建全局比对框架。其他许多则是局限于特定区域的部分基因组序列，这些序列可用于比较来自明确流行病学背景和/或病理状况的更多分离株。大多数研究表明，B19病毒的基因变异性较低，分子流行病学仅在有限的地理和时间范围内可行，并且基因组序列与独特的病理和临床表现之间不存在明显的相关性。最近，已鉴定出几种病毒分离株，它们与参考序列相比显示出显著的序列多样性。变异分离株的鉴定增加了对该病毒组基因多样性的了解，并使得能够鉴定出三个不同的基因簇，它们彼此之间有大约10%的差异，并且与其他细小病毒仍有明显区别，可以被认为是人类红细胞病毒组内的不同基因型，并且显示出清晰的系统发育关系。这些变异分离株提出了一些有趣的问题，涉及人类红细胞病毒基因变异的实际程度、这些病毒在流行病学方面的相关性以及它们在红细胞病毒相关疾病发病机制中的可能影响。
