Williams C E, Collier C C, Sardesai N, Ohm H W, Cambron S E
Crop Production and Pest Control Research Unit USDA-ARS-MWA and Department of Entomology, Purdue University, West Lafayette, IN 47907, USA.
Theor Appl Genet. 2003 Nov;107(8):1516-23. doi: 10.1007/s00122-003-1393-y. Epub 2003 Aug 20.
A new source of resistance to the highly virulent and widespread biotype L of the Hessian fly, Mayetiola destructor (Say), was identified in an accession of tetraploid durum wheat, Triticum turgidum Desf., and was introgressed into hexaploid common wheat, Triticum aestivum L. Genetic analysis and deletion mapping revealed that the common wheat line contained a single locus for resistance, H31, residing at the terminus of chromosome 5BS. H31 is the first Hessian fly-resistance gene to be placed on 5BS, making it unique from all previously reported sources of resistance. AFLP analysis identified two markers linked to the resistance locus. These markers were converted to highly specific sequence-tagged site markers. The markers are being applied to the development of cultivars carrying multiple genes for resistance to Hessian fly biotype L in order to test gene pyramiding as a strategy for extending the durability of deployed resistance.
在四倍体硬粒小麦（Triticum turgidum Desf.）的一个种质中鉴定出了对麦秆蝇（Mayetiola destructor (Say)）高毒力且广泛分布的生物型L的一种新抗性来源，并将其导入到六倍体普通小麦（Triticum aestivum L.）中。遗传分析和缺失作图表明，普通小麦品系含有一个抗性单基因座H31，位于5BS染色体末端。H31是第一个定位在5BS上的麦秆蝇抗性基因，使其与所有先前报道的抗性来源不同。AFLP分析鉴定出两个与抗性基因座连锁的标记。这些标记被转化为高度特异性的序列标签位点标记。这些标记正被应用于培育携带多个抗麦秆蝇生物型L基因的品种，以测试基因聚合作为一种延长已部署抗性持久性的策略。
