利用AFLP标记评估中国黄槿（锦葵科）的遗传多样性。
Genetic diversity of Hibiscus tiliaceus (Malvaceae) in China assessed using AFLP markers.
作者信息
Tang Tian, Zhong Yang, Jian Shuguang, Shi Suhua
机构信息
Key Laboratory of Gene Engineering of the Ministry of Education, Zhongshan University, Guangzhou 510275, People's Republic of China.
出版信息
Ann Bot. 2003 Sep;92(3):409-14. doi: 10.1093/aob/mcg156.
Amplified fragment length polymorphism (AFLP) markers were used to investigate the genetic variations within and among nine natural populations of Hibiscus tiliaceus in China. DNA from 145 individuals was amplified with eight primer pairs. No polymorphisms were found among the 20 samples of a marginal population of recent origin probably due to a founder effect. Across the other 125 individuals, 501 of 566 bands (88.5%) were polymorphic, and 125 unique AFLP phenotypes were observed. Estimates of genetic diversity agreed with life history traits of H. tiliaceus and geographical distribution. AMOVA analysis revealed that most genetic diversity resided within populations (84.8%), which corresponded to results reported for outcrossing plants. The indirect estimate of gene flow based on phiST was moderate (Nm=1.395). Long-distance dispersal of floating seeds and local environments may play an important role in shaping the genetic diversity of the population and the genetic structure of this species.
扩增片段长度多态性（AFLP）标记被用于研究中国9个黄槿自然种群内部及种群间的遗传变异。用8对引物对145个个体的DNA进行了扩增。近期起源的边缘种群的20个样本中未发现多态性，这可能是由于奠基者效应。在其他125个个体中，566条带中的501条（88.5%）具有多态性，并且观察到了125种独特的AFLP表型。遗传多样性估计值与黄槿的生活史特征及地理分布相符。分子方差分析（AMOVA）表明，大部分遗传多样性存在于种群内部（84.8%），这与异交植物的报道结果一致。基于phiST的基因流间接估计值适中（Nm = 1.395）。漂浮种子的远距离传播和当地环境可能在塑造该种群的遗传多样性及该物种的遗传结构方面发挥重要作用。
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