酿酒酵母S期期间Rad52非依赖性的联合环状微型染色体积累
Rad52-independent accumulation of joint circular minichromosomes during S phase in Saccharomyces cerevisiae.
作者信息
Wellinger Ralf Erik, Schär Primo, Sogo Jose M
机构信息
Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland.
出版信息
Mol Cell Biol. 2003 Sep;23(18):6363-72. doi: 10.1128/MCB.23.18.6363-6372.2003.
Abstract
We investigated the formation of X-shaped molecules consisting of joint circular minichromosomes (joint molecules) in Saccharomyces cerevisiae by two-dimensional neutral/neutral gel electrophoresis of psoralen-cross-linked DNA. The appearance of joint molecules was found to be replication dependent. The joint molecules had physical properties reminiscent of Holliday junctions or hemicatenanes, as monitored by strand displacement, branch migration, and nuclease digestion. Physical linkage of the joint molecules was detected along the entire length of the minichromosome and most likely involved newly replicated sister chromatids. Surprisingly, the formation of joint molecules was found to be independent of Rad52p as well as of other factors associated with a function in homologous recombination or in the resolution of stalled replication intermediates. These findings thus imply the existence of a nonrecombinational pathway(s) for the formation of joint molecules during the process of DNA replication or minichromosome segregation.
摘要
我们通过补骨脂素交联DNA的二维中性/中性凝胶电泳，研究了酿酒酵母中由联合环状微型染色体（联合分子）组成的X形分子的形成。发现联合分子的出现依赖于复制。通过链置换、分支迁移和核酸酶消化监测发现，联合分子具有类似于霍利迪连接体或半连环体的物理性质。在微型染色体的整个长度上检测到联合分子的物理连接，并且很可能涉及新复制的姐妹染色单体。令人惊讶的是，发现联合分子的形成独立于Rad52p以及与同源重组功能或停滞复制中间体的解决相关的其他因素。因此，这些发现意味着在DNA复制或微型染色体分离过程中存在联合分子形成的非重组途径。
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