Runfola Matteo, Rossi Simone, Panunzi Simona, Spada Pier Luigi, Gui Daniele
Department of Surgery, Catholic University, Rome, Italy.
BMC Gastroenterol. 2003 Sep 10;3:26. doi: 10.1186/1471-230X-3-26.
Botulinum toxin blocks acetylcholine release from nerve endings and acts as a long term, reversible inhibitor of muscle contraction as well as of salivary, sweat gland, adrenal and prostatic secretions. The aim of the present study is to investigate whether gastric submucosal injection of botulinum toxin type A reduces stimulated gastric production of HCl.
Sixty-four rats were randomized in two groups and laparotomized. One group was treated with botulinum toxin-A 10 U by multiple submucosal gastric injections, while the second group was injected with saline. Two weeks later, acid secretion was stimulated by pyloric ligation and acid output was measured. Body weight, food and water intake were also recorded daily.
HCl production after pyloric ligation was found to be significantly lower in botulinum toxin-treated rats (657 +/- 90.25 micromol HCl vs. 1247 +/- 152. P = 0.0017). Botulinum toxin-treated rats also showed significantly lower food intake and weight gain.
Botulinum toxin type A reduces stimulated gastric acidity. This is likely due either to inhibition of the cholinergic stimulation of gastric parietal cells, or to an action on the myenteric nervous plexuses. Reduction of growth and food intake may reflect both impaired digestion and decreased gastric motility.
肉毒杆菌毒素可阻断神经末梢乙酰胆碱的释放，是肌肉收缩以及唾液、汗腺、肾上腺和前列腺分泌的长效可逆抑制剂。本研究旨在探讨胃黏膜下注射A型肉毒杆菌毒素是否能减少刺激引起的胃酸分泌。
64只大鼠随机分为两组并进行剖腹手术。一组通过多次胃黏膜下注射10 U A型肉毒杆菌毒素进行治疗，而另一组注射生理盐水。两周后，通过幽门结扎刺激胃酸分泌并测量酸排出量。每天还记录体重、食物和水的摄入量。
发现幽门结扎后，接受肉毒杆菌毒素治疗的大鼠胃酸分泌显著降低（657±90.25微摩尔盐酸 vs. 1247±152。P = 0.0017）。接受肉毒杆菌毒素治疗的大鼠食物摄入量和体重增加也显著降低。
A型肉毒杆菌毒素可降低刺激引起的胃酸度。这可能是由于抑制了胃壁细胞的胆碱能刺激，或者是对肌间神经丛的作用。生长和食物摄入量的减少可能反映了消化功能受损和胃动力下降。
