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检测巨细胞病毒抗体的四种技术的比较

Comparison of four techniques for detection of antibodies to cytomegalovirus.

作者信息

Kraat Y J, Hendrix R M, Landini M P, Bruggeman C A

机构信息

Department of Medical Microbiology, University of Limburg, Academic Hospital Maastricht, The Netherlands.

出版信息

J Clin Microbiol. 1992 Feb;30(2):522-4. doi: 10.1128/jcm.30.2.522-524.1992.

DOI:10.1128/jcm.30.2.522-524.1992
PMID:1311337
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265094/
Abstract

Four serological methods were compared and evaluated for use in detecting cytomegalovirus antibody in blood and organ donors. Western blotting (immunoblotting), latex agglutination, enzyme-linked immunosorbent assay, and a recent available microparticle enzyme immunosorbent assay were used. The microparticle enzyme immunoassay appears to compare favorably with each of the other three assays tested for screening blood and organ donors for a previous cytomegalovirus infection.

摘要

对四种血清学方法进行了比较和评估,以用于检测血液和器官捐献者中的巨细胞病毒抗体。使用了蛋白质印迹法(免疫印迹法)、乳胶凝集试验、酶联免疫吸附测定法以及最近可用的微粒酶免疫测定法。微粒酶免疫测定法与所测试的其他三种方法相比,在筛查血液和器官捐献者既往是否感染巨细胞病毒方面似乎具有优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c7d/265094/c575e3e15d81/jcm00026-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c7d/265094/c575e3e15d81/jcm00026-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9c7d/265094/c575e3e15d81/jcm00026-0279-a.jpg

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Comparison of four techniques for detection of antibodies to cytomegalovirus.检测巨细胞病毒抗体的四种技术的比较
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J Clin Pathol. 1997 Jan;50(1):59-63. doi: 10.1136/jcp.50.1.59.
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J Clin Microbiol. 1994 Apr;32(4):981-6. doi: 10.1128/jcm.32.4.981-986.1994.

本文引用的文献

1
Transfusion-associated cytomegalovirus infections.输血相关巨细胞病毒感染
Rev Infect Dis. 1983 Nov-Dec;5(6):977-93. doi: 10.1093/clinids/5.6.977.
2
Application of denatured, electrophoretically separated, and immobilized lysates of herpes simplex virus-infected cells for detection of monoclonal antibodies and for studies of the properties of viral proteins.将单纯疱疹病毒感染细胞的变性、经电泳分离并固定的裂解物用于单克隆抗体检测及病毒蛋白特性研究。
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3
Enzyme-linked immunosorbent assay for measurement of antibody against cytomegalovirus and rubella virus in a single serum dilution.
酶联免疫吸附测定法用于在单一血清稀释度下检测抗巨细胞病毒和风疹病毒抗体。
J Clin Pathol. 1981 Jun;34(6):665-9. doi: 10.1136/jcp.34.6.665.
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Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus.使用过氧化物酶标记抗原的直接酶联免疫吸附测定法,用于检测巨细胞病毒免疫球蛋白M抗体。
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Evaluation of a commercial latex agglutination test for detecting antibodies to cytomegalovirus in organ donors and transplant recipients.评估一种用于检测器官捐献者和移植受者中巨细胞病毒抗体的商业乳胶凝集试验。
J Virol Methods. 1987 May;16(1-2):13-9. doi: 10.1016/0166-0934(87)90026-7.
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Serum antibodies to individual cytomegalovirus structural polypeptides in renal transplant recipients during viral infection.肾移植受者病毒感染期间针对个体巨细胞病毒结构多肽的血清抗体
Microbiol Immunol. 1986;30(7):683-95. doi: 10.1111/j.1348-0421.1986.tb02994.x.
7
Cytomegalovirus immune globulin and seronegative blood products to prevent primary cytomegalovirus infection after marrow transplantation.巨细胞病毒免疫球蛋白和血清阴性血液制品预防骨髓移植后原发性巨细胞病毒感染。
N Engl J Med. 1986 Apr 17;314(16):1006-10. doi: 10.1056/NEJM198604173141602.
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Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting.
J Med Virol. 1985 Dec;17(4):303-11. doi: 10.1002/jmv.1890170403.
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Comparison of six methods for the detection of antibody to cytomegalovirus.六种检测巨细胞病毒抗体方法的比较
J Clin Microbiol. 1985 Dec;22(6):1014-9. doi: 10.1128/jcm.22.6.1014-1019.1985.
10
Detection of cytomegalovirus antibody with latex agglutination.用乳胶凝集法检测巨细胞病毒抗体。
J Clin Microbiol. 1985 Jul;22(1):68-70. doi: 10.1128/jcm.22.1.68-70.1985.