González José M, Covert Joseph S, Whitman William B, Henriksen James R, Mayer Frank, Scharf Birgit, Schmitt Rüdiger, Buchan Alison, Fuhrman Jed A, Kiene Ronald P, Moran Mary Ann
Departamento de Microbiología y Biología Celular, Facultad de Farmacia, Universidad de La Laguna, 38071 La Laguna, Tenerife, Spain.
Departments of Marine Sciences, University of Georgia, Athens, GA 30602, USA.
Int J Syst Evol Microbiol. 2003 Sep;53(Pt 5):1261-1269. doi: 10.1099/ijs.0.02491-0.
Three Gram-negative, rod-shaped, aerobic bacteria that were capable of degrading dimethylsulfoniopropionate (DMSP) were isolated from marine waters. These isolates (DSS-3(T), DSS-10 and ISM(T)) exhibited the ability to demethylate and cleave DMSP, as well as to degrade other sulfur compounds related to DMSP that are cycled in marine environments. Intracellular poly-beta-hydroxybutyrate inclusions, surface blebs and one polar, complex flagellum that rotated exclusively in the clockwise direction were observed for DSS-3(T). The outer membrane of ISM(T) was separated from the cytoplasm at the poles in a toga-like morphology. The primary fatty acid in both strains was C(18 : 1)omega7c. DNA G+C contents for the isolates were 68.0+/-0.1, 68.1+/-0.1 and 66.0+/-0.2 mol% for DSS-3(T), DSS-10 and ISM(T), respectively. 16S rRNA gene sequence analyses placed these organisms within the Roseobacter lineage of the alpha-PROTEOBACTERIA: Closely related species were Silicibacter lacuscaerulensis and Ruegeria atlantica (DSS-3(T) and DSS-10) and Roseovarius tolerans (ISM(T)). Neither DSS-3(T) nor ISM(T) exhibited 16S rRNA similarity >97 % or DNA-DNA hybridization values >45 % to their nearest described relatives. Genotypic and phenotypic analyses support the creation of two novel species: Silicibacter pomeroyi sp. nov. with strain DSS-3(T) (=ATCC 700808(T)=DSM 15171(T)) as the type strain, and Roseovarius nubinhibens sp. nov. with strain ISM(T) (=ATCC BAA-591(T)=DSM 15170(T)) as the type strain.
从海水中分离出三株革兰氏阴性、杆状、需氧且能够降解二甲基巯基丙酸内盐（DMSP）的细菌。这些分离菌株（DSS-3(T)、DSS-10和ISM(T)）具有使DMSP脱甲基和裂解的能力，以及降解海洋环境中与DMSP循环相关的其他含硫化合物的能力。观察到DSS-3(T)含有细胞内聚-β-羟基丁酸包涵体、表面泡以及一根仅沿顺时针方向旋转的极生、复杂鞭毛。ISM(T)的外膜在两极处与细胞质分离，呈托加样形态。这两株菌株的主要脂肪酸均为C(18 : 1)ω7c。分离菌株的DNA G+C含量分别为：DSS-3(T)为68.0±0.1 mol%，DSS-10为68.1±0.1 mol%，ISM(T)为66.0±0.2 mol%。16S rRNA基因序列分析将这些微生物归为α-变形菌纲的玫瑰杆菌属谱系：亲缘关系较近的物种有湖蓝硅细菌和大西洋吕杰氏菌（DSS-3(T)和DSS-10）以及耐盐玫瑰色菌（ISM(T)）。DSS-3(T)和ISM(T)与它们最近的已描述亲缘种的16S rRNA相似性均不超过97%，DNA-DNA杂交值均不超过45%。基因型和表型分析支持创建两个新物种：以菌株DSS-3(T)（=ATCC 700808(T)=DSM 15171(T)）为模式菌株的波默罗伊硅细菌新种，以及以菌株ISM(T)（=ATCC BAA-591(T)=DSM 15170(T)）为模式菌株的抑制性玫瑰色菌新种。
