Brice S L, Stockert S S, Jester J D, Huff J C, Bunker J D, Weston W L
Department of Dermatology, University of Colorado School of Medicine, Denver.
J Am Acad Dermatol. 1992 Apr;26(4):594-8. doi: 10.1016/0190-9622(92)70087-v.
Although herpes simplex virus (HSV) has been detected in the peripheral blood of immunocompromised patients and in neonates with disseminated disease, the extent to which this virus may be present in the blood during a localized infection in otherwise healthy adults is unknown.
The purpose of this study was to determine whether HSV may be detected in the peripheral blood during acute recurrent herpes labialis.
Peripheral blood mononuclear cells (PBMCs) were obtained from otherwise healthy adults with recurrent herpes labialis, both during an acute episode and several weeks after the lesions had healed. The PBMCs were examined for the presence of HSV with the polymerase chain reaction (PCR) and viral culture.
By PCR, HSV DNA was detected in 7 of 34 specimens from an acute episode but in none of 24 specimens in the convalescent stage (p less than 0.004). PBMCs from seven donors, who were seronegative for HSV, were also negative for HSV by PCR. Viral cultures of 22 PBMC specimens were negative (including four specimens that were positive by PCR).
The presence of HSV DNA in the blood is a transient phenomenon limited to the period of active infection in a minority of patients with herpes labialis, although it may be important in the development of disseminated disease as well as in the pathogenesis of herpes-associated cutaneous processes such as erythema multiforme.
尽管在免疫功能低下患者的外周血以及患有播散性疾病的新生儿中已检测到单纯疱疹病毒（HSV），但在其他方面健康的成年人发生局部感染期间，该病毒在血液中的存在程度尚不清楚。
本研究的目的是确定在急性复发性唇疱疹期间外周血中是否能检测到HSV。
从患有复发性唇疱疹的其他方面健康的成年人中获取外周血单个核细胞（PBMC），在急性发作期间以及皮损愈合数周后采集。通过聚合酶链反应（PCR）和病毒培养检测PBMC中HSV的存在情况。
通过PCR，在急性发作期的34份标本中有7份检测到HSV DNA，但在恢复期的24份标本中均未检测到（p<0.004）。来自7名HSV血清学阴性供体的PBMC经PCR检测HSV也为阴性。22份PBMC标本的病毒培养均为阴性（包括4份PCR阳性标本）。
血液中HSV DNA的存在是一种短暂现象，仅限于少数唇疱疹患者的活跃感染期，尽管它在播散性疾病的发生以及如多形红斑等疱疹相关皮肤病变的发病机制中可能也很重要。
