猪呼吸道冠状病毒（PRCV）对猪的实验性感染：病毒排泄量的测定
Experimental infection of pigs with the porcine respiratory coronavirus (PRCV): measure of viral excretion.
作者信息
Bourgueil E, Hutet E, Cariolet R, Vannier P
机构信息
Ministère de l'Agriculture et de la Forêt, CNEVA Laboratoire de Recherches Avicole et Porcine, Ploufragan, France.
出版信息
Vet Microbiol. 1992 Apr;31(1):11-8. doi: 10.1016/0378-1135(92)90136-h.
Abstract
Twelve pigs were experimentally infected with a porcine respiratory coronavirus (PRCV) by the oronasal route. Viral excretion was measured daily by two means-deep nasal swabs and air samples obtained in a cyclone sampler. Clinical signs were very slight on infected pigs. Airborne virus could be recovered from day 1 to day 6 post-infection in the cyclone sampler as well as in petri dishes placed in the same loose-box. Viral titres obtained from nasal swabs were significantly correlated with those obtained from air samples. Different collection media were compared. The most efficient media for the collection of infectious viral particles contained a protective agent such as foetal calf serum.
摘要
通过口鼻途径对12头猪进行猪呼吸道冠状病毒（PRCV）实验性感染。每天通过两种方式测量病毒排泄物——深部鼻拭子和在旋风采样器中采集的空气样本。感染猪的临床症状非常轻微。在感染后的第1天至第6天，在旋风采样器以及放置在同一宽松猪舍中的培养皿中均可检测到空气传播病毒。从鼻拭子获得的病毒滴度与从空气样本获得的病毒滴度显著相关。比较了不同的采集介质。用于采集传染性病毒颗粒的最有效介质含有一种保护剂，如胎牛血清。
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