Haro J M, Sabio J M, Vargas F
Departamento de Bioquímica, Facultad de Medicina, Granada, Spain.
J Endocrinol Invest. 1992 Sep;15(8):605-8. doi: 10.1007/BF03344933.
The number of beta-adrenoceptors was measured in cardiac and renal cortex membranes from controls and thyroxine-treated rats. Two methods of membrane preparation (sucrose- and KCl-extraction) were compared. KCl-extraction yielded a higher number of beta-adrenoceptors, accompanied by increased activity of the plasma membrane enzyme marker 5'-nucleotidase. Thyroxine increased the number of beta-adrenoceptors in both cardiac and renal cortex membranes prepared with both methods. These results suggest that: i) Enrichment of the membrane fraction may explain the higher number of beta-adrenoceptors found with KCl, ii) Thyroid hormone plays a role in the regulation of beta-adrenoceptors in the renal cortex.
在对照组和甲状腺素处理的大鼠的心脏和肾皮质膜中测量β-肾上腺素能受体的数量。比较了两种膜制备方法（蔗糖提取和氯化钾提取）。氯化钾提取产生的β-肾上腺素能受体数量更多，同时伴有质膜酶标志物5'-核苷酸酶活性增加。甲状腺素增加了用这两种方法制备的心脏和肾皮质膜中β-肾上腺素能受体的数量。这些结果表明：i）膜组分的富集可能解释了用氯化钾发现的较高数量的β-肾上腺素能受体，ii）甲状腺激素在肾皮质中β-肾上腺素能受体的调节中起作用。
