Stumpf W E, Bidmon H J, Li L, Pilgrim C, Bartke A, Mayerhofer A, Heiss C
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
Histochemistry. 1992 Oct;98(3):155-64. doi: 10.1007/BF00315874.
Autoradiograms were prepared from midbrains and hindbrains of male and female Siberian hamsters (Phodopus sungorus), kept under short-day or long-day illumination, after injection of tritium-labeled 1,25-dihydroxycholecalciferol (vitamin D, soltriol). Concentration and retention of radioactivity was noted in nuclei of certain neurons, glial cells, and ependymal cells, and in choroid epithelium. Labeled neurons of varying intensity were found throughout the brainstem in distinct populations at characteristic topographical sites, which include cranial nerve motor nuclei, the nucleus (n.) reticularis tegmenti pontis, the caudoventral region of the n. raphe dorsalis, the n. trapezoides, the n. vestibularis lateralis and n. vestibularis superior, neurons in the various nuclei of the sensory trigeminus, accessory optic nuclei, scattered neurons in nuclei of the reticular formation, the n. ambiguus, certain cells in the area postrema, and many others. Glial cells with nuclear labeling, probably microglia, were scattered predominantly in or near myelinated nerve fascicles. The choroid epithelium showed strong nuclear labeling throughout the ventricle. Nuclear labeling of ependyma was variable and weak, mainly at ventral and lateral extensions (recesses) of the ventricle. The extensive presence of nuclear binding in select neural structures indicates that vitamin D exerts specific genomic effects on cell populations that are known to be involved in the regulation of motor, sensory, autonomic, neuroendocrine, metabolic, and immune functions. The results of these studies, in conjunction with those from other brain and peripheral tissues, recognize vitamin D-soltriol as a steroid hormone with a wide scope of hormone-specific target cells, similar to estrogen, androgen, and adrenal steroids, and which are topographically distinct and characteristic for its functions as the steroid hormone of sunlight.
对处于短日照或长日照光照条件下的雄性和雌性西伯利亚仓鼠（Phodopus sungorus）注射氚标记的1,25 - 二羟胆钙化醇（维生素D，骨化三醇）后，制备其大脑中脑和后脑的放射自显影片。在某些神经元、神经胶质细胞和室管膜细胞的细胞核以及脉络丛上皮中，观察到放射性的浓度和保留情况。在脑干各处不同强度的标记神经元分布于特征性的地形部位，包括脑神经运动核、脑桥被盖网状核、中缝背核的尾腹侧区域、斜方体核、外侧前庭核和上前庭核、感觉三叉神经各核中的神经元、副视核、网状结构核中的散在神经元、疑核、最后区的某些细胞以及许多其他细胞。带有核标记的神经胶质细胞，可能是小胶质细胞，主要散在于有髓神经束内或其附近。脉络丛上皮在整个脑室中显示出强烈的核标记。室管膜的核标记变化不定且较弱，主要位于脑室的腹侧和外侧延伸部（隐窝）。在特定神经结构中广泛存在的核结合表明，维生素D对已知参与运动、感觉、自主、神经内分泌、代谢和免疫功能调节的细胞群体发挥特定的基因组效应。这些研究结果，连同来自其他脑和外周组织的结果，确认骨化三醇是一种类固醇激素，具有广泛的激素特异性靶细胞，类似于雌激素、雄激素和肾上腺类固醇，并且在地形上具有独特性和特征性，作为阳光的类固醇激素发挥其功能。
