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近交系果蝇个体中转座元件的基因组分布。

Genomic distribution of transposable elements among individuals of an inbred Drosophila line.

作者信息

Di Franco C, Galuppi D, Junakovic N

机构信息

Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Roma, Italy.

出版信息

Genetica. 1992;86(1-3):1-11. doi: 10.1007/BF00133706.

DOI:10.1007/BF00133706
PMID:1334902
Abstract

The stability of the elements of eleven transposon families (412, B 104, blood, 297, 1731, G, copia, mdg 4, hobo, jockey and I) has been compared by the Southern technique among individuals of a Drosophila line that has been subjected to 30 generations of sister sib matings. The 412, B104, blood, 297, 1731 and G elements appear stable. Heterochromatic copia and hobo elements and euchromatic I elements appear highly polymorphic. In addition, copia, mdg 4, jockey and I elements undergo an instability resulting in significant variations in relative intensity among autoradiographic bands. The extent of the polymorphisms detected strongly suggests de novo rearrangements of transposable elements.

摘要

通过Southern技术,比较了11个转座子家族(412、B104、blood、297、1731、G、copia、mdg 4、hobo、jockey和I)的元件在经过30代姐妹同胞交配的果蝇品系个体中的稳定性。412、B104、blood、297、1731和G元件看起来是稳定的。异染色质的copia和hobo元件以及常染色质的I元件看起来高度多态。此外,copia、mdg 4、jockey和I元件会发生不稳定性,导致放射自显影片带之间的相对强度出现显著变化。检测到的多态性程度强烈表明转座元件发生了从头重排。

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