Singh I P, Coppenhaver D H, Chopra A K, Baron S
Department of Microbiology, University of Texas Medical Branch, Galveston.
Viral Immunol. 1992 Winter;5(4):293-303. doi: 10.1089/vim.1992.5.293.
A broadly active antiviral glycoprotein (UTI beta) occurs naturally in human sera at an average antiviral titer of 50 U/ml. This inhibitor is active against all virus families tested to date, including representative poxviruses, herpesviruses, enteroviruses, paramyxoviruses, alpha-viruses, flaviviruses, bunyaviruses, and rhabdoviruses. It is a glycoprotein of approximately 60,000 +/- 10,000 Da, which is stable at pH 2 to 10 and at 80 degrees C for up to 10 min. Mild oxidation with NaIO4 and treatment with glycosidases inactivates the material. Proteolytic degradation of the inhibitor molecule releases small active components of < 1000 Da, which retain antiviral activity. This activity of the small components has increased heat stability (120 degrees C for 15 min) and is inactivated by glycosidases. The antiviral activity thus appears to reside mainly in the oligosaccharide moiety of the glycoprotein. The inhibitor does not neutralize virions, but prevents attachment of most viruses to cells. These properties occur also in highly purified preparations. These findings indicate that human serum contains significant concentrations of a broadly active antiviral glycoprotein, which is distinct from interferon and other antiviral substances naturally found in human body fluids and tissues.
一种具有广泛活性的抗病毒糖蛋白（UTIβ）天然存在于人类血清中，平均抗病毒效价为50 U/ml。这种抑制剂对迄今测试的所有病毒科均有活性，包括代表性的痘病毒、疱疹病毒、肠道病毒、副粘病毒、甲病毒、黄病毒、布尼亚病毒和弹状病毒。它是一种分子量约为60,000 +/- 10,000 Da的糖蛋白，在pH 2至10以及80℃下可稳定存在长达10分钟。用高碘酸钠进行轻度氧化以及用糖苷酶处理会使该物质失活。抑制剂分子的蛋白水解降解会释放出分子量小于1000 Da的小活性成分，这些成分保留抗病毒活性。这些小成分的这种活性具有更高的热稳定性（120℃下15分钟），并且会被糖苷酶灭活。因此，抗病毒活性似乎主要存在于糖蛋白的寡糖部分。该抑制剂不会中和病毒粒子，但会阻止大多数病毒附着于细胞。这些特性在高度纯化的制剂中也存在。这些发现表明，人血清中含有高浓度的具有广泛活性的抗病毒糖蛋白，它不同于人体体液和组织中天然存在的干扰素及其他抗病毒物质。
